Taconic Transgenic Exchange

Mrp2

CONSTITUTIVE KNOCK OUT

Licensing Requirements

FVB Background

Model Number	Zygosity	Nomenclature
6621-F	Homozygous	FVB.129P2-Abcc2 ^tm1AhsN7
6621-M	Homozygous	FVB.129P2-Abcc2 ^tm1AhsN7

Model Description

This targeted mutation strain carries a disruption of the Abcc2 gene that encodes multidrug resistance protein 2
Useful for the examination of compound uptake and elimination mechanisms
Useful for studies on drug resistance in tumors

Origin:
The Mrp2 mouse was developed in the laboratory of Alfred Schinkel of the Netherlands Cancer Institute. The model was created through targeting of the Abcc2 gene in 129/Ola-derived E14 ES cells and injecting the targeted cells into C57BL/6 blastocysts. Resultant chimeras were backcrossed to FVB/N for seven generations (N7). Taconic received stock in 2006, and the line was embryo transfer derived. The colony is maintained by mating homozygotes.

Color: Albino

Genetics: Wildtype for Nnt mutation; carries Pde6b^rd1 mutation

Initial Publication:
Vlaming MLH, Mohrmann K, Wagenaar E, de Waart DR, Oude Elferink RPJ, Lagas JS, van Tellingen O, Vainchtein LD, Rosing H, Beijnen JH, Schellens JHM, Schinkel AH. (2006) Carcinogen and anticancer drug transport by Mrp2 in vivo: studies using Mrp2 (Abcc2) knockout mice. J Pharmacol Exp Ther 318(1):319-327

Additional Publication:
van Waterschoot RAB, ter Heine R, Waagenar E, van der Kruijssen CMM, Rooswinkel RW, Huitema ADR, Beijnen JH, Schinkel AH (2010). Effects of cytochrome P450 3A (CYP3A) and the drug transporters P-glycoprotein (MDR1/ABCB1) and MRP2 (ABCC2) on the pharmacokinetics of lopinavir. Br J Pharmacol 160(5):1224-1233.

Animal Diet: NIH #31M Rodent Diet

Licensing Requirements:
Label License: Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, and grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:

Title to these Models and biological materials derived from them remains with Taconic Farms, Inc
The Models will be used for research purposes only
The Models will not be bred except to obtain embryos or fetuses required for research purposes
The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes

Taconic Transgenic Models are produced in Isolated Barrier Unit (IBU™) facilities under MPF™ conditions, and shipped in Taconic Transit Cages (TTC™).