Removal of Animals from a Taconic Germ Free Shipper

1. Withdraw vinyl shipper from the cardboard box (use care to keep cages and oval filter upright).
   
   
2. Open end of connecting sleeve:
   1. Unfold sleeve and remove yellow vinyl tape that was folded and sealed prior to shipment.
   2. When open the sleeve is compatible with a standard 12" diameter Isolator port.
   
   
3. Swab inside of sleeve and the face of the Mylar diaphragm with a sterilant (using caution not to apply too much pressure to Mylar diaphragm).
   
   
4. Pull sleeve partway onto the swabbed port of the destination Isolator, spray sleeve portion of shipper with sterilant by puckering the sleeve around the nozzle of the atomizer and spraying until the sleeve is inflated.
   
   
5. Secure with tape, to make a complete tape seal of the shipper to the destination Isolator port.
   
   
6. When ready remove the yellow tape from the circumference of the Mylar diaphragm.
   
   
7. Carefully, work the Mylar diaphragm to a horizontal position (using external manipulation).
   
   
8. Pull the diaphragm through the port and into the Isolator.
   
   
9. Rotate the cages and slide toward the Isolator port (using external pressure).
   
   
10. Pull in cages and remove the bands that are securing the cages lids and remove animals.