- Contains a disruption of the endogenous murine apolipoprotein E (ApoE) gene
- ApoE, a glycoprotein, is a structural component of very low density lipoprotein (vLDL) synthesized by the liver and intestinally synthesized chylomicrons
- ApoE is also a constituent of a subclass of high density of lipoproteins (HDL) involved in cholesterol transport
- ApoE mediates high affinity binding of chylomicrons and vLDL particles to the LDL receptor, allowing for specific uptake of these particles by the liver, preventing the accumulation of cholesterol rich particles in the plasma
- Homozygous ApoE mice are devoid of apoE protein
- Mice develop normally, but exhibit five times normal serum plasma cholesterol and spontaneous atherosclerotic lesions
- Studies indicate a role for ApoE in immune system regulation, nerve regeneration, and muscle differentiation
- Useful in studying the role of apoE in lipid metabolism, atherogenesis, and nerve injury and to investigate intervention therapies that modify the atherogenic process
- This line carries the Gpi1a allele and the ES1a and ES1b alleles in contrast to the C57BL/6N inbred which is Gpi1b and ES1a
- Approximately 0.6% of mice in our APOE colony exhibit malocclusion. Animals with malocclusion are euthanized. Links between atherosclerosis and periodontal disease have been reported in humans and it is possible that these mice develop periodontal disease that leads to inflammation, gum disease, and subsequently malocclusion. To reduce the incidence of malocclusion, Taconic recommends providing animals with autoclavable chew sticks
Origin: The ApoE mouse was developed in the laboratory of Nobuya Maeda at the University of North Carolina. The model was created by targeting the Apoe gene in E14TG2a ES cells and injecting the targeted cells into C57BL/6J blastocysts. Resultant chimeras were backcrossed for ten generations (N10) and intercrossed to homozygosity. Taconic received stock in May 1998 and backcrossed one additional generation (N11). The line was derived by embryo transfer and is maintained by incrossing homozygous mice.
Genetics: Wild type for Nnt mutation
Initial Publication: Piedrahita JA, Zhang SH, Hagaman JR, Oliver PM, Maeda N. (1992) Generation of mice carrying a mutant apolipoprotein E gene inactivated by gene targeting in embryonic stem cells. Proc Natl Acad Sci USA, 89(10):4471-5.
Label License: Conditions of Use for Taconic Transgenic Models™
- Title to these Models and biological materials derived from them remains with Taconic Farms, Inc
- The Models will be used for research purposes only
- The Models will not be bred except to obtain embryos or fetuses required for research purposes
- The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
Taconic Transgenic Models are produced in Isolated Barrier Unit (IBU™) facilities under MPF™ conditions, and shipped in Taconic Transit Cages (TTC™).