Apoe (ApoE)

Model Description

• Contains a disruption of the endogenous murine apolipoprotein E (ApoE) gene
• ApoE, a glycoprotein, is a structural component of very low density lipoprotein (vLDL) synthesized by the liver and intestinally synthesized chylomicrons
• ApoE is also a constituent of a subclass of high density of lipoproteins (HDL) involved in cholesterol transport
• ApoE mediates high affinity binding of chylomicrons and vLDL particles to the LDL receptor, allowing for specific uptake of these particles by the liver, preventing the accumulation of cholesterol rich particles in the plasma
• Homozygous ApoE mice are devoid of apoE protein
• Mice develop normally, but exhibit five times normal serum plasma cholesterol and spontaneous atherosclerotic lesions
• Studies indicate a role for ApoE in immune system regulation, nerve regeneration, and muscle differentiation
• Useful in studying the role of apoE in lipid metabolism, atherogenesis, and nerve injury and to investigate intervention therapies that modify the atherogenic process
• This line carries the Gpi1a allele and the ES1a and ES1b alleles in contrast to the C57BL/6N inbred which is Gpi1b and ES1a

Origin: The ApoE mouse was developed in the laboratory of Nobuya Maeda at the University of North Carolina. The model was created by targeting the Apoe gene in E14TG2a ES cells and injecting the targeted cells into C57BL/6J blastocysts. Resultant chimeras were backcrossed for ten generations (N10) and intercrossed to homozygosity. Taconic received stock in May 1998 and backcrossed one additional generation (N11). The line was derived by embryo transfer and is maintained by incrossing homozygous mice.

Color: Black

Genetics: Wild type for Nnt mutation

Initial Publication: Piedrahita JA, Zhang SH, Hagaman JR, Oliver PM, Maeda N. (1992) Generation of mice carrying a mutant apolipoprotein E gene inactivated by gene targeting in embryonic stem cells. Proc Natl Acad Sci USA, 89(10):4471-5.

Animal Diet:

• NIH #31M Rodent Diet at U.S Facilities

Licensing Requirements:
Label License: Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions of Sale and the following terms of use:

• Title to these Models and biological materials derived from them remains with Taconic Farms, Inc
• The Models will be used for research purposes only
• The Models will not be bred except to obtain embryos or fetuses required for research purposes
• The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.

Taconic Transgenic Models are produced in Isolated Barrier Unit (IBU™) facilities under MPF™ conditions, and shipped in Taconic Transit Cages (TTC™).

Related Reports

View the production locations(s) and the accompanying current IHMS^TM Health Report and Taconic Health Standard for this model

Phenotyping

• Histologic Examination of Arteries
• Phenotyping Data for Apoe-M mice

Price Table

APOE (in U.S. Dollars)

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