An anesthetized and surgically prepared animal is positioned under a dissecting scope in dorsal recumbency. A 2cm ventral cervical skin incision is made right of the midline with its caudal terminus at the level of the clavicle. Underlying salivary and lymphatic tissues are separated by means of blunt dissection to visualize the right common jugular vein. Five millimeters of vessel cranial to the site where the jugular vein passes under the clavicle is mobilized. A sterile JVC is inserted into the vessel and secured in place with suture. A 0.5cm midline skin incision is made between the scapulae. Hemostats are used to draw the JVC by the port back through the scapular incision. Skin incisions are closed and the cannula port is secured with a stainless steel wound clip. The port protrudes through the center of the clip and the ports anchoring bead should remain under the skin.
Cannula material consists of sterile polyethylene (PE) tubing with a silicone rubber intra-vascular tip. The access port consists of a 25mm length of PE50 tubing (0.023" ID) that is sealed with a sterile stainless steel pin. 23 gauge blunted needles are required to access the port. Fill volume of the cannula is 20ul.
Heparinized Glycerol (500 IU/ml): 10.0 mL stock heparin (1000 IU/mL) + 10.0 mL 99% Glycerol solution (Sigma).
Patency is verified by the ability to withdraw a blood sample within 24 hours of shipment and is guaranteed upon animal receipt. To maintain animals over longer periods of time, cannulas need to be flushed twice a week (once every 3-4 days). Cannulas can be flushed by following the sampling procedure below minus the withdrawal of the whole blood sample. Please access our web site for details on in-house patency studies.
Contact Us or call: