Anesthetic: Isoflurane
An anesthetized and surgically prepared animal is positioned under a dissecting scope in dorsal recumbency. A 2cm ventral skin incision is made along the crease formed by the abdomen and right thigh. Blunt dissection of the adductor muscles is used to visualize the right femoral vein. Five to ten millimeters of vessel is mobilized and a sterile FVC is inserted into the vessel and secured in place with suture. A 0.5cm dorsal, midline skin incision is made between the scapulae. Hemostats are used to draw the FVC by the port back through the scapular incision. Skin incisions are closed and the cannula port is secured with a stainless steel wound clip.
Cannula material consists of a single length (230 mm) of sterile microrenethane tubing with a 40 mm intra-vascular tip and 25 mm flared access port. The access port is sealed with a sterile stainless steel pin. 23 gauge blunted needles are required to enter the port. Fill volume of the cannula is 60ul.
Heparinized Glycerol (500 IU/ml): 10.0 mL stock heparin (1000 IU/mL) + 10.0 mL 99% Glycerol solution (Sigma).
Patency is verified by the ability to withdraw a blood sample within 24 hours of shipment and is guaranteed upon animal receipt. To maintain animals over longer periods of time, cannulas need to be flushed twice a week (once every 3-4 days). Cannulas can be flushed by following the sampling procedure below minus the withdrawal of the whole blood sample. Please access our web site for details on in-house patency studies.
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