Model No. | Nomenclature | Genotype |
---|---|---|
1175 | B6.129S2-Cd4tm1Nik Tg(CD4) N7 | Inquire for genotype |
Nomenclature: B6.129S2-Cd4tm1Nik Tg(CD4) N7
Origin
Dr. Toshika Sakihama and colleagues in the Ellis Reinherz laboratory at Dana-Farber Cancer Institute made this human CD4 transgenic model on the murine Cd4 knockout background. A human CD4 molecule with a point mutation (Phe to Ile at amino acid residue 43) in the C’ strand residue of domain 1 (a region essential for binding MHC class II molecules) was generated by site-directed mutagenesis. The transgene was injected into fertilized eggs of mixed stock, and resultant transgene-positive mice were backcrossed to Cd4 knockout mice (Taconic model #1055) for 14 generations. The Cd4/hCD4 mice were received at Taconic in September 1999 and embryo transfer derived. The colony was maintained by breeding homozygous/hemizygous Cd4/hCD4 mice with homozygous Cd4 knockout mice to produce homozygous/hemizygous Cd4/hCD4 mice.
This model is cryopreserved and available for recovery. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Purchase of this model includes perpetual use rights and a deliverable of four mutant animals at the Murine Pathogen Free™ health standard along with a genotyping protocol. For models which include a recombinase gene or multiple alleles, all alleles will be provided, but individual animals may not contain all mutant alleles.
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Initial Publication:
Sakihama T, Hunsicker ME, Hussey RE, Reinherz EL; Human CD4 residue Phe 43 is critical for repertoire development and maturation of MHC class II restricted CD4 single-positive T lineage cells in vivo; Eur J Immunol 2000 30:279-290. Note: The transgene with the point mutation (F43I) described in this publication is inserted on the X chromosome. The line received at Taconic has the same mutant hCD4, however, it is not linked to the X chromosome. The phenotype, class II MHC restriction and dependence are the same as described in the publication.
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Item | Commercial | Nonprofit |
---|---|---|
Cryopreserved Model | $29,400.00 | $21,000.00 |
Cryopreserved models are invoiced upon shipment of recovered animals. Once orders are placed, the full purchase price will be applied if the order is canceled. For orders greater than 4 animals, please contact Taconic for options.
Fees for Taconic Transit Cages™ and freight are in addition to the price above.
Item | Commercial | Nonprofit |
---|---|---|
Cryopreserved Model | €26.250,00 | €19.425,00 |
Cryopreserved models are invoiced upon shipment of recovered animals. Once orders are placed, the full purchase price will be applied if the order is canceled. For orders greater than 4 animals, please contact Taconic for options.
Fees for Taconic Transit Cages™ and freight are in addition to the price above.
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