Nomenclature: C57BL/6-Gh^{tm1.1 mrl}

• The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
• Exon 1 contains the translation initiation codon.
• Exons 1-5 (complete gene) and 1333 bp of sequence upstream of exon 1 (promoter region) have been flanked by loxP sites (size of loxP-flanked region: 3.3 kb).
• The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
• The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
• The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 1 to 5 (including 1333 bp of sequence upstream of exon 1) will result in loss of function of the Gh gene by deleting the complete gene.
• The remaining recombination sites are located in non-conserved regions of the genome.
• Datamining and Design performed in 2009.

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