| Model No. | Nomenclature | Genotype |
|---|---|---|
| 21617 | C57BL/6-Piezo1tm1.1Tac | Inquire for genotype |
Piezo1 Conditional KI-PM Mouse
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Overview
Nomenclature: C57BL/6-Piezo1tm1.1Tac
- The model carries a conditional knock-in point mutation (cKI-PM) in the Piezo 1 (piezo-type mechanosensitive ion channel component 1).
- In absence of Cre, the model is expected to express the wildtype Piezo 1 (Fam38a) allele.
- Upon crossbreeding with a Cre model, the constitutive KI-PM allele may be obtained with resulting expression of the mutated Piezo 1 R2482H protein.
Availability
Cryopreserved at TaconicOrigin
The Piezo1 mouse was developed at Taconic Biosciences on behalf of Scripps Health in 2012. The model was generated using BAC clones from the C57BL/6J RPCIB-731 BAC library and was transfected into the Taconic C57BL/6NTac ES cell line. Homologous recombinant clones were isolated using double positive (NeoR and PuroR) selection. The conditional KI-PM allele was obtained after Flp-mediated removal of the selection markers. Taconic received donor animals from Scripps in 2023 and the model is stored as homozygous sperm.
Source: Taconic Biosciences
Genetics
Guides & Publications
Initial Publication:
- Shang Ma, Stuart Cahalan, Gregory LaMonte, Nathan D. Grubaugh, Weizheng Zeng, Swetha E. Murthy, Emma Paytas, Ramya Gamini, Viktor Lukacs, Tess Whitwam, Meaghan Loud, Rakhee Lohia, Laurence Berry, Shahid M. Khan, Chris J. Janse, Michael Bandell, Christian Schmedt, Kai Wengelnik, Andrew I. Su, Eric Honore, Elizabeth A. Winzeler, Kristian G. Andersen, Ardem Patapoutian. Common PIEZO1 Allele in African Populations Causes RBC Dehydration and Attenuates Plasmodium Infection. Cell, Volume 173, Issue 2, 2018. Pages 443-455.e12. ISSN 0092-8674, https://doi.org/10.1016/j.cell.2018.02.047
- Shang Ma, Adrienne E. Dubin, Yunxiao Zhang, Seyed Ali Reza Mousavi, Yu Wang, Adam M. Coombs, Meaghan Loud, Immacolata Andolfo, Ardem Patapoutian, A role of PIEZO1 in iron metabolism in mice and humans, Cell, Volume 184, Issue 4, 2021, Pages 969-982.e13, ISSN 0092-8674, https://doi.org/10.1016/j.cell.2021.01.024
- Hill, R.Z., Loud, M.C., Dubin, A.E. et al. PIEZO1 transduces mechanical itch in mice. Nature 607, 104–110 (2022). https://doi.org/10.1038/s41586-022-04860-5
- Jesse R Holt, Wei-Zheng Zeng, Elizabeth L Evans, Seung-Hyun Woo, Shang Ma, Hamid Abuwarda, Meaghan Loud, Ardem Patapoutian, Medha M Pathak (2021) Spatiotemporal dynamics of PIEZO1 localization controls keratinocyte migration during wound healing eLife 10:e65415. https://doi.org/10.7554/eLife.65415
- Nakamichi R, Ma S, Nonoyama T, Chiba T, Kurimoto R, Ohzono H, Olmer M, Shukunami C, Fuku N, Wang G, Morrison E, Pitsiladis YP, Ozaki T, D'Lima D, Lotz M, Patapoutian A, Asahara H. The mechanosensitive ion channel PIEZO1 is expressed in tendons and regulates physical performance. Sci Transl Med. 2022 Jun;14(647):eabj5557. doi: 10.1126/scitranslmed.abj5557. Epub 2022 Jun 1. PMID: 35648809
Transit, Housing & Welfare
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Licensing
To purchase this EZcohort model, sign the Terms of Sale (choose from the list below) and email it directly to legal@taconic.com:
This model is sold under terms which grant perpetual use rights.
This model is sold under terms which grant perpetual use rights.
Pricing - USD
21617
| Item | Commercial | Nonprofit |
|---|---|---|
| Cryopreserved Model | $23,100.00 | $17,325.00 |
Cryopreserved models are invoiced upon shipment of recovered animals. Once orders are placed, the full purchase price will be applied if the order is canceled. For orders greater than 4 animals, please contact Taconic for options.
Fees for Taconic Transit Cages™ and freight are in addition to the price above.
Pricing - EUR
21617
| Item | Commercial | Nonprofit |
|---|---|---|
| Cryopreserved Model | €21.000,00 | €15.855,00 |
Cryopreserved models are invoiced upon shipment of recovered animals. Once orders are placed, the full purchase price will be applied if the order is canceled. For orders greater than 4 animals, please contact Taconic for options.
Fees for Taconic Transit Cages™ and freight are in addition to the price above.
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