Nomenclature: C57BL/6-Acly^{tm1.1 Mrl}

• The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
• Acly exon 2 contains the translation initiation codon.
• Exons 17-19 have been flanked by loxP sites.
• The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
• The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.
• Deletion of exons 17-19 should result in loss of function of the Acly gene by abolishing the essential binding domains.
• The remaining recombination sites are located in intron regions of the genome.
• The constitutive KO allele is available as line 10412.
• Data mining and Design performed in 2004.

Availability