Taconic’s Cryopreserved Repository offers quick speed to cohort, ease of access for high quality study-ready models within 12 weeks of purchase, internationally accredited health testing, and integrates seamlessly into Taconic’s complete solution to provide you with the animals you need for your research studies.
Taconic Quality
Easily import directly into Taconic barrier or your own vivarium with internationally accredited IHMS health testing and various health standard options. All advertised models in our Cryopreserved Repository are available as cryopreserved stock for fast access to your gene of interest, whether you're in the U.S. or Europe.
Competitive Pricing
Including discounted model pricing if you choose to breed your recovered model with Taconic. The price you see on our website includes licensing, recovery, and genotyping of offspring. Competitive shipping prices available based on location.
Easy Licensing
Taconic makes licensing cryopreserved models easy, and only requires a single signature for perpetual use of the cryopreserved model that best suits your needs. Simply sign the Terms of Sale (TOS) from the model’s webpage and send the signed TOS & and PO over to Taconic’s legal team or your Taconic sales representative.
Taconic has redefined what to expect from model generation and colony management services by providing complete stewardship of the process while delivering the most comprehensive and flexible services in the industry.
Our ultimate goal is to facilitate providing you with the model that best suits your research needs to drive advancements in human health. If you cannot find the model you are looking for, Taconic has the most experienced model generation team in the industry and can craft the mouse or rat model that is best suited for your research studies with our fully licensed gene modification toolkit and decades of scientific expertise. Once your model has been generated, you can seamlessly transition to breeding for cohort production at your ideal scale on your desired timeline.
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Perpetual licensing agreements that never expire
Internationally accredited
health standards
Basic and Comprehensive data packages available for most models.
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Upon recovery of a cryopreserved model, customers can choose to pursue custom cohort production through Taconic’s colony management solutions. Taconic offers a variety of add-on services such as Animal Identification, Body Weight Tracking, Custom Diet & Water Administration, Murine Biospecimen Collections, Surgical Services, etc. Taconic Biosciences' ecosystem of support including Scientific Program Managers, Project Managers, and Application Scientists are assigned from the moment we start planning your colony and stay with the project until completion. Our flexible fee-for-service project plans allow you to change your colony management strategy at any time, and our online eTaconic® platform ensures that your team will always have full visibility of your colony.
The Knockout Respository (KOR) collection consists of over 4,000 fully licensed knockout mouse models generated by Lexicon Genetics, Inc. via gene targeting and gene trapping. Most lines are on a mixed 129S5 x C57BL/6J-Tyrc-Brd background. Comprehensive Phenotypic Data Packages are available for purchase for Knockout Repository lines.
The Knockout Repository models utilize proprietary embryonic stem (ES) cells bearing a genetically engineered mutation. Mutant ES cells are obtained through two complementary, proven technologies: Gene Targeting and Gene Trapping.
Gene targeting by homologous recombination in mouse ES cells enables generation of highly specific alterations in targeted genes. It is a widely used and versatile method for the generation of knockout mouse lines.
Targeting vectors are constructed either via the aid of a phage murine 129/SvEv genomic library, or directly via long-range PCR methods. The final targeting vectors typically contain around 10 kilobases of overall sequence homology flanking the deleted region for the gene of interest. An ES cell positive selection cassette, usually containing a LacZ expression marker, is also used in combination with negative selection cassettes in the targeting vector backbones. These technologies, along with the power of using isogenic DNA, allow high average targeting frequencies. Gene targeting was used to generate approximately half of the existing knockout mouse lines available.
Gene trapping is a high throughput method of generating knockout mouse clones. It is a method of random mutagenesis by which the insertion of a DNA element into endogenous genes leads to transcriptional disruption. Unlike gene targeting by homologous recombination, a single gene trap vector can be used to mutate thousands of individual genes in mouse ES cells, as well as enable the rapid identification of the mutated genes.
The retroviral gene trap vectors contain a splice acceptor sequence followed by a promoter-less selectable marker. Insertion of the retroviral vector into an expressed gene leads to the splicing of the endogenous upstream exons into this cassette to generate a fusion transcript. The vectors also contain a promoter that is active in ES cells, followed by a first exon (such as that of the Bruton's Tyrosine Kinase gene, BTK) upstream of a splice donor signal. Splicing from this signal to the exons downstream of the insertion gives rise to a fusion transcript that can be used to generate a sequence tag of the trapped gene. The BTK gene also contains termination codons in all reading frames to prevent translation of downstream fusion transcripts.
The knockout models are designed to be null alleles. Due to compensatory genetic mechanisms, knockout status cannot be guaranteed and will have to be confirmed by the customer. Some validation data can be found in our phenotypic data packages.
Understanding the phenotype associated with a targeted or gene trapped mutation is essential for identifying and selecting drug targets or studying symptoms of human diseases. Taconic enables you to jumpstart your research by offering off-the-shelf phenotypic data packages for all of the Knockout Repository lines. These phenotypic data were collected at the time that the line was generated. Cohorts of animals were developed and tested in a comprehensive battery of assays that were fully validated with benchmark knockouts and/or drugs to confirm their reliability and reproducibility. Phenotypic assays are focused on six main disease areas:
The Genetically Engineered Models (GEM) Collection offers access to several hundred proprietary GEM lines, generated by a pharma company, primarily on a C57BL/6 genetic background. The collection contains conditional and constitutive knockout (KO) mice, transgenic over-expressing lines, and conditional and constitutive targeted transgenic lines. These lines are available to non-profit researchers at a subsidized price.
* In rare cases involving models with recombinases or multiple genetic modifications, this deliverable may be reduced to 1-2 mice.
Murine Pathogen Free™ (MPF™). Mice at a more restrictive health standard may be delivered upon request and at additional cost.
The customer will receive at least 4 mutant mice. In rare cases that the cryopreserved material also contains a recombinase gene, this may be reduced to 1-2 mutant mice.
No. Cryopreserved materials are the property of Taconic or the Sponsor and may not be provided directly to customers.
Taconic cannot warranty individual mice for fertility. Mice are provided within the optimal breeding age range. Heterozygous mutants are less likely to have fertility problems compared to homozygous mice. Taconic recommends that customers consider doing at least one round of expansion by breeding the received heterozygous mice to wild type prior to intracrossing to homozygosity to reduce the chance of problems propagating the mice received. Taconic will discuss environmental and husbandry factors with the customer to determine whether there may be an external problem.
Customers must inform Taconic in the event the mice die during transit or within 24 hours of receipt. Taconic will attempt to replace the shipment using mice available. Customers should unpack and inspect all shipments of mice immediately after arrival. Mice that arrive dead or in poor health should be reported within 3 days by the receiving institution. Taconic does not have control over housing and environmental conditions in recipient facilities and thus cannot be held responsible for mice that die more than 24 hours after receipt.
The price shown on the model page is due upon completion of cryorecovery. Any additional services such as a RapidEXPANSION™ are an additional service which will be billed in addition to the basic package.
The GEM Collection Terms of Sale permit breeding, crossbreeding and other further modifications of the model.
Once the appropriate agreement has been executed and the customer has provided a PO, the timeline is approximately 14-16 weeks until live mice are ready for shipment. Mice are provided at 6-12 weeks, which is the optimal breeding age.
The GEM Collection Terms of Sale grant perpetual use rights to commercial customers. Any models previously distributed under an academic MTA are subject to term restrictions in that MTA.
