Nomenclature: C57BL/6-Irf5 ^{tm1.1 Mrl}

• The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6N Tac ES cell line.
• Irf5 exon 1 contains the translation initiation codon.
• Exons 3 to 9 have been flanked by loxP sites (size of loxP-flanked region 4.2 kb).
• A polyadenylation signal (hGHpA: human Growth Hormone polyadenylation signal) has been inserted 3' of the Irf5 gene in order to prevent downstream transcription in the constitutive KO allele.
• The conditional KO allele has been obtained after Flp-mediated removal of the selection marker by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
• The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
• Deletion of exons 3 to 9 should result in loss of function of the Irf5 gene by removing most of the gene. After Cre-mediated deletion of exons 3 to 9, the hGHpA cassette should prevent downstream transcription.
• The remaining recombination sites are located in non-conserved regions of the genome.
• The corresponding constitutive KO allele is available as line 12685.
• Datamining and Design performed in 2012.

Availability