| Model No. | Nomenclature | Genotype |
|---|---|---|
| 9166 | C57BL/6-Ahrtm1.2Arte | Inquire for genotype |
Ahr Knockout Mouse
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Overview
Nomenclature: C57BL/6-Ahrtm1.2Arte
- This targeted mutation strain carries a deletion of the mouse aryl hydrocarbon receptor (Ahr) gene.
- Useful in prediction of CYP induction and therefore pharmacokinetics, drug toxicity and efficacy in humans.
- Mice which are homozygous knockouts for the Ahr gene are smaller than heterozygotes or wild types. As described in the literature, dead newborn and missing litters are common in Ahr knockout animals, with some incidence of death after weaning also seen. Taconic recommends that this line be ordered no earlier than 6 weeks of age, and 8-10 weeks of age or older may be preferable, particularly if transit times will be longer than 2 days.
- The Ahr Knockout mouse has immune system abnormalities. Taconic recommends maintaining this model under more restrictive husbandry conditions to minimize clinical problems.
Origin
The Ahr Knockout mouse was developed by Taconic in collaboration with CXR Biosciences. It was generated by crossing an AHR humanized mouse line (Model 9165) with a Cre deleter mouse. The Humanized AHR Mouse model was created through a targeted replacement of mouse Ahr Exon 3 with a cDNA construct containing human AHR Exons 3-11 in C57BL/6NTac-derived ES cells. Targeted ES cells were injected into BALB/cJBomTac blastocysts and the resultant chimeras were backcrossed to a Flpe deleter strain on C57BL/6J to eliminate selection markers. The Ahr Knockout Mouse model was derived from the Humanized AHR Mouse line through a Cre-mediated deletion of the human AHR sequence. Mouse exon 3 is deleted in the knockout, resulting in an out of frame splicing of exons 2 to exon 4. The absence of functional Ahr protein was proven experimentally. Taconic received stock in 2009, and the line was embryo transfer derived. The colony was maintained by mating homozygotes.
This model is cryopreserved and available for recovery. Models can typically be recovered and delivered to customers within 12 weeks after order receipt. Purchase of this model includes perpetual use rights and a deliverable of four mutant animals at the Murine Pathogen Free™ health standard along with a genotyping protocol. For models which include a recombinase gene or multiple alleles, all alleles will be provided, but individual animals may not contain all mutant alleles.
Taconic’s Colony Management experts can design a plan to grow your colony faster.
Genetics
Guides & Publications
Initial Publication:
Not published yet.
Applications & Therapeutic Areas
- ADMET
- Vaccine Research
- Autoimmune Disease
- Immunology
- Infectious Disease
- Inflammation
- Metabolic Disease
- Microbiome
- Oncology & Immuno-Oncology
- Safety Assessment
Transit, Housing & Welfare
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Diet
- Licensing
- Pricing - USD
- Pricing - EUR
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Ahr Knockout Mouse
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.
This model is sold under terms which grant perpetual use rights.
This model is sold under terms which grant perpetual use rights.
Pricing - USD
9166
| Item | Commercial | Nonprofit |
|---|---|---|
| Cryopreserved Model | $21,000.00 | $13,000.00 |
Cryopreserved models are invoiced upon shipment of recovered animals. Once orders are placed, the full purchase price will be applied if the order is canceled. For orders greater than 4 animals, please contact Taconic for options.
Fees for Taconic Transit Cages™ and freight are in addition to the price above.
Pricing - EUR
9166
| Item | Commercial | Nonprofit |
|---|---|---|
| Cryopreserved Model | €21.000,00 | €13.000,00 |
Cryopreserved models are invoiced upon shipment of recovered animals. Once orders are placed, the full purchase price will be applied if the order is canceled. For orders greater than 4 animals, please contact Taconic for options.
Fees for Taconic Transit Cages™ and freight are in addition to the price above.
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