Nomenclature: C57BL/6-Lair1 ^{tm1.2 Mrl}

• The targeting vector has been generated using C57BL/6J DNA and transfected into Taconic’s C57BL/6N Tac ES cell line.
• Lair1 exon 1 contains the translation initiation codon.
• Exons 3 to 7 have been replaced by the FRT site flanked neomycin resistance gene cassette.
• The neomycin resistance gene cassette has been removed by transient transfection of Flp recombinase into targeted ES cell clones.
• The constitutive KO line has been derived using C57BL/6NTac animals.
• Deletion of exons 3 to 7 should result in loss of function of the Lair1 gene by removing most of the coding sequence.
• The remaining recombination sites are located in non-conserved regions of the genome.
• Datamining and Design performed in 2005.

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