Nomenclature: C57BL/6-Gt(ROSA)26Sor ^{tm1(CAG-Nod2)Mrl}

• The targeting vector has been transfected into TaconicArtemis C57Bl/6 ES cell line.
• The following elements have been inserted into the ROSA26 locus:
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  • A CAG promoter cassette.
  • A loxP-flanked transcription termination cassette (STOP) containing a combination of polyadenylation signals.
  • The Nod2 ORF together with a Kozak sequence (GCCACC).
  • The hGH polyadenylation signal and an additional polyadenylation signal.
  
  
• The conditional TTG line has been generated by crossing chimeras to C57BL/6NTac animals.
• The constitutive TTG allele can be generated by Cre-mediated recombination by crossing line 12910 to a Cre-Deleter.
• Deletion of the hGHpA cassette should result in transcription of the Nod2 cDNA.
• The presence of the NeoR cassette will terminate the transcription from the ROSA26 promoter.
• Datamining and Design performed in 2012.

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