Nomenclature: C57BL/6-Prmt2^{tm1.2 Mrl}

• The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
• Prmt2 exon 2 contains the translation initiation codon.
• Exon 5 has been flanked by loxP sites and contains the mutation residues 145-149 GCGTG to AAAAA.
• The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
• The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
• Deletion of exon 5 should result in loss of function of the Prmt2 by deleting part of the SAM_bd domain.
• The remaining recombination sites are located in intron regions of the genome.
• Data mining and Design performed in 2006.