Nomenclature: C57BL/6-Ffar4^tm1.1Mrl

• The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.
• Exon 1 contains the translation initiation codon.
• Exon 1 and approx. 1.5 kb of sequence upstream of exon 1 (promoter region) have been flanked by loxP sites (size of loxP-flanked region: 3.2 kb).
• The positive selection marker (Puromycin resistance - PuroR) has been flanked by FRT sites and inserted into intron 1.
• The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
• The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
• The constitutive KO allele can be generated by Cre-mediated recombination.
• Deletion of exon 1 and proximal promoter should result in loss of function of the Ffar4 gene, by preventing transcription of the Ffar4 mRNA and by deleting the transmembrane domains 1 to 4.
• The remaining recombination sites will be located in non-conserved regions of the genome.
• Datamining and Design performed 2014.