Nomenclature: C57BL/6-Fgfr1 ^{tm1.1 Mrl}

• The targeting vector has been generated using C57BL/6J DNA and has been transfected into TaconicArtemis C57BL/6N Tac ES cell line.
• Fgfr1 exon 2 contains the translation initiation codon.
• Exons 4 and 5 have been flanked by loxP sites (size of loxP-flanked region 1.6 kb).
• The conditional KO allele has been obtained after Flp-mediated removal of the selection marker by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
• The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
• Deletion of exons 4 and 5 should result in the loss of function of the Fgfr1 gene by deleting half of the second Ig-like C2-type domain, and by generating a frameshift from exon 3 to exon 6 (premature Stop codon in exon 6).
• Remaining recombination sites are located in non-conserved regions of the genome.
• Datamining and Design performed in 2010.

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