| Model No. | Nomenclature | Genotype |
|---|---|---|
| 10501 | FVB/N-Tg(Gfap-luc)53Xen | Inquire for genotype |
Gfap-luc
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Overview
Nomenclature: FVB/N-Tg(Gfap-luc)53Xen
- Carries a 12 kb fragment of Glial fibrillary acidic protein (Gfap) promoter, 850 bp human β-globin intron 2, and modified firefly luciferase cDNA (Promega pGL-3).
- Basal expression of the reporter is observed primarily in brain. Basal expression of the reporter in non-treated male and female was observed in ear, paw, and tail, and does not change during treatment. The reporter is inducible following injury to the CNS.
- This model provides for the rapid study of transcriptional regulation of the Gfap gene and indications of possible neural injury.
- The Gfap-luc LPTA® animal model is useful in studying changes in the health of nerve tissue, primarily the CNS, resulting in Gfap gene regulation. Changes such as those caused by physical trauma, chemical treatment, and bacterial infections resulting in injury or changes in pressure within the cerebral spinal fluid are most likely to induce Gfap-luc expression.
Origin
The Gfap-luc mouse was developed by Caliper Life Sciences. The model was created by microinjecting a transgene containing a fragment of the glial fibrillary acidic protein promoter, a human β-globin intron 2 and modified firefly luciferase cDNA from pGL3. This transgene was microinjected into FVB/N zygotes. The resultant mice from founder line 53 were bred to FVB/NTac mice and intracrossed to homozygosity. Taconic received stock from Caliper in 2010. The line was embryo transfer derived. The line was maintained by mating homozygous male and female mice.
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