Nomenclature: C57BL/6-Grik1 ^{tm1.1 Mrl}

• The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6N Tac ES cell line.
• Grik1 exon 1 contains the translation initiation site.
• Exon 4 has been flanked by LoxP sites.
• The conditional KO allele has been obtained after Flp-mediated removal of the selection marker by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
• The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
• Deletion of exon 4 (1.0 kb) should result in loss of function of the Grik1 gene by generating a frameshift from exon 3 to exons 5 to 12 (premature Stop codon in exon 5).
• Remaining recombination sites are located in non-conserved regions of the genome.
• Datamining and Design performed in 2009.

Availability