Nomenclature: C57BL/6-Kcnj1 ^{tm1.2 Mrl}

• The targeting vector has been generated using C57BL/6J DNA and has been transfected into TaconicArtemis C57BL/6N Tac ES cell line.
• Kncj1 exon 2 contains the complete open reading frame (ORF).
• Exon 2 has been flanked by loxP sites (size of loxP-flanked region 3.5 kb).
• The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
• The constitutive KO line has been derived using C57BL/6NTac animals and the Cre-transgene was removed by segregation.
• Deletion of exon 2 should result in loss of function of the Kncj1 gene by deleting the complete ORF and by removing the 3'UTR.
• The remaining recombination sites are located in non-conserved regions of the genome.
• The corresponding conditional KO allele is available as line 11481.
• Datamining and Design performed in 2011.

Availability