Nomenclature: C57BL/6-Nlrp3 ^{tm1.1 Mrl}

• The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6N Tac ES cell line.
• Nlrp3 exon 2 contains the translation initiation codon.
• Exon 4 has beeen flanked by loxP sites (size of loxP-flanked region 2.6 kb).
• The conditional KO allele has been obtained after Flp-mediated removal of the selection marker by crossing chimeras to a Flp-Deleter on a C57BL/6 background.
• The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.
• Deletion of exon 4 should result in the loss of function of the Nlrp3 gene by deleting the NACHT domain and by generating a frameshift from exon 3 to all downstream exons (premature Stop codon in exon 5).
• The remaining recombination sites are located in non-conserved regions of the genome.
• The corresponding constitutive KO allele is available as line 12935.
• Datamining and Design performed in 2012.

Availability