| Model No. | Nomenclature | Genotype |
|---|---|---|
| 11269 | C57BL/6-Tg(Camk2a-PLA2G4A)9 Mrl | Inquire for genotype |
Pla2g4 - Model 11269 - TG
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Overview
Nomenclature: C57BL/6-Tg(Camk2a-PLA2G4A)9 Mrl
- The transgenic construct contains 5´ the 8.5 kb mouse CaM-kinase II alpha chain (mCamk2a) promoter fragment, an 850 bp DNA fragment containing the human b-globin intron 2, and 2247bp ORF of human PLA2G4A cDNA (GenBank Acc# NM_024420), followed by 221bp of SV40 poly A sequence at the 3´ end in a pGL3 vector backbone (Promega).
- A consensus KOZAK sequence has been added upstream of the starter ATG.
- The plasmid backbone was removed and the transgenic construct was microinjected in C57BL/6 mice by pronuclear injection.
- Putative transgenic founders were identified by genotyping and bred for germline transmission with C57BL/6 mice.
- Heterozygous transgenic mice were identified by genotyping.
- Line 11266, C57BL/6-Tg(Camk2a-PLA2G4A)31 Mrl, has the highest expression level of the transgene in the brain.
- Line 11269, C57BL/6-Tg(Camk2a-PLA2G4A)9 Mrl, has the medium expression level of the transgene in the brain.
- Datamining and Design performed in 2009.
Availability
Cryopreserved at TaconicSource: Xenogen
This model is cryopreserved and available for recovery. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Purchase of this model includes perpetual use rights and a deliverable of four mutant animals at the Murine Pathogen Free™ health standard along with a genotyping protocol. For models which include a recombinase gene or multiple alleles, all alleles will be provided, but individual animals may not contain all mutant alleles.
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Genetics
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Transit, Housing & Welfare
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Pla2g4 - Model 11269 - TG
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.
The GEM Collection Commercial and Academic/Non-Profit Terms of Sale grant perpetual use rights.
| Item | Commercial | Nonprofit |
| Cryopreserved Model | $32,500 | $5,500 |
| Item | Commercial | Nonprofit |
| Cryopreserved Model | €29.500 | €5.005 |
Cryopreserved models are invoiced upon shipment of recovered animals. Once orders are placed, the full purchase price will be applied if the order is canceled. For orders greater than 4 animals, please contact Taconic for options.
Fees for Taconic Transit Cages™ and freight are in addition to the price above.
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