Nomenclature: C57BL/6-Vnn1^{tm1.2 Mrl}

• The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.
• Vnn1 exon 1 contains the translation initiation codon.
• Exon 4 has been flanked by loxP sites.
• The constitutive KO allele has been generated after Cre-mediated recombination by crossing chimeras to a Cre-Deleter on a C57BL/6 background.
• The constitutive KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Cre-transgene was removed by segregation.
• Deletion of exon 4 should result in loss of function of the Vnn1 gene by deleting the C-term of Carbon-nitrogen hydrolase domain.
• The remaining recombination sites are located in intron regions of the genome.
• Data mining and Design performed in 2006.

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