| Model No. | Nomenclature | Genotype |
|---|---|---|
| 601-F | C.129S6(B6)-Rag2tm1Fwa N12 | ko/ko |
| 601-M | C.129S6(B6)-Rag2tm1Fwa N12 | ko/ko |
Rag2 - Model 601
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- Description
- Data
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- Health Report
- Overview
- Genetics
- Guides & Publications
- Applications & Therapeutic Areas
- Transit, Housing & Welfare
- Diet
Overview
Nomenclature: C.129S6(B6)-Rag2tm1Fwa N12
- Contains a disruption of the recombination activating gene 2 (Rag2)
- Homozygous mice exhibit total inability to initiate V(D)J rearrangement and fail to generate mature T or B lymphocytes.
- Useful for vaccine development, transplantation studies and hematopoiesis research.
- Carries the H2d haplotype
Origin
The Rag2 mouse was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells and injecting the targeted cells into blastocysts.
Taconic received stock from the Ellis Reinherz lab at the Dana Farber Cancer Institute in 1993 as heterozygotes on a mixed B6;129S6 background. The mice were embryo transfer derived and intercrossed to generate homozygotes. Backcrossing to BALB/cAnNTac began at Taconic in September 1993, sponsored by Dana Farber Cancer Institute and screened by S. Koyasu. Backcrossing to N12 was completed in March 1997. The colony is maintained by incrossing homozygous mice.
Genetics
Guides & Publications
Initial Publication:
Shinkai, Y, Rathbun, G, Lam, KP, Oltz, EM, Stewart, V, Mendelsohn, M, Charron, J, Datta, M, Young, F, Stall, AM, and Alt, FW. (1992) RAG-2-Deficient Mice Lack Mature Lymphocytes Owing to Inability to Initiate V(D)J Rearrangement, Cell, 68: 855-867.
Applications & Therapeutic Areas
- Cell and Tissue Humanized
- Immunology
- Inflammation
- Oncology & Immuno-Oncology
Transit, Housing & Welfare
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All 601 mice are packed as cagemates, without recombination of animals from different cages into a single Taconic Transit Cage (TTC). We recommend that customers do NOT recombine 601 mice from different Taconic Transit Cages into the same cage at or after unpacking.
Packing Practices
Taconic standard practice is to recombine animals of different home cages and/or ages from a single model and sex during packing, except in specific cases where Taconic's animal welfare policy prohibits recombination due to aggression or other concerns. When an order is fulfilled with animals from more than one week of birth, this standard practice results in animals from a range of birth weeks packed together in a single TTC. When an order is fulfilled with animals from genotyped models, this standard practice results in animals from different home cages packed together in a single TTC.
Customers who wish to keep animals from different weeks of birth separated should place orders with the special instruction "Divide and label by age." Note that this special request can result in increased costs for additional Taconic Transit Cages, dividers and/or freight charges.
Taconic discourages other types of custom packing requests as they can have a negative impact on animal welfare. Learn more.
Diet
Data
Rag2 Physiological Data Summary (February 2005; N=10/group):
| Parameter | Units | 601(M) Males | 601(M) Females |
|---|---|---|---|
| Serum Chemistry | Avg ± S.D. | ||
| Calcium | mg/dL | 10.2 ± 0.3 | 11.1 ± 0.2 |
| Phosphorous | mg/dL | 8.0 ± 1.6 | 9.5 ±1.2 |
| Glucose | mg/dL | 132 ± 26 | 86 ± 17 |
| Creatinine | mg/dL | 0.2 ± 0.0 | 0.1 ± 0 |
| BUN | mg/dL | 22 ± 4 | 18 ± 2 |
| Total Bilirubin | mg/dL | 0.1 ± 0.0 | 0.1 ± 0 |
| ALK | U/L | 161 ± 13 | 105 ± 73 |
| ALT U/L | U/L | 52 ± 13 | 40 ± 9 |
| Total Protein | g/dL | 5.3 ± 1.6 | 5.4 ± 0.2 |
| Blood Counts | |||
| Eryhtron | |||
| Red Blood Cells | X10^6/uL | 10.278 ± 0.605 | 9.986 ± 0.329 |
| Nucleated RBC | X10^6/uL | 0.0 | 0.0 |
| Hemoglobin | g/dL | 16.8 ± 1.0 | 16.2 ± 0.6 |
| Hematocrit | % | 50.3 ± 2.9 | 48.8 ± 1.5 |
| MCV | fL | 49 ± 0 | 49 ± 1 |
| MCH | pg | 16.3 ± 0.2 | 16.2 ± 0.2 |
| MCHC | % | 33.4 ± 0.4 | 33.2 ± 0.3 |
| Platelets | |||
| Platelets | X10^3/uL | 1225 ± 217 | 1086 ± 112 |
| Leukogram | |||
| White Blood Cells | x10^3/uL | 2.26 ± 1.148 | 1.187 ± 0.679 |
| Neutrophil | x10^3/uL | 1.772 ± 0.884 | 0.826 ± 0.484 |
| Bands | x10^3/uL | 0.0 | 0.0 |
| Lymphocyte | x10^3/uL | 0.427 ± 0.244 | 0.311 ± 0.174 |
| Monocytes | x10^3/uL | 0.037 ± 0.040 | 0.020 ± 0.012 |
| Eosinophil | x10^3/uL | 0.024 ± 0.014 | 0.030 ± 0.022 |
| Basophils | x10^3/uL | 0.0 | 0.0 |
| Organ Weights | |||
| Body Weight (Fasted) | g | 23.16 ± 2.11 | 16.78 ± 0.99 |
| Stomach | g | 0.225 ± 0.035 | 0.210 ± 0.027 |
| Ileum | g | 0.853 ± 0.161 | 0.693 ± 0.111 |
| Colon | g | 0.218 ± 0.049 | 0.195 ± 0.029 |
| Lungs | g | 0.273 ± 0.041 | 0.227 ± 0.016 |
| Heart | g | 0.218 ± 0.027 | 0.145 ± 0.019 |
| Liver | g | 1.326 ± 0.168 | 0.944 ± 0.070 |
| Spleen/Pancreas | g | 0.245 ± 0.048 | 0.169 ± 0.023 |
| Kidney (L) | g | 0.240 ±0.024 | 0.140 ± 0.018 |
| Kidney (R) | g | 0.242 ± 0.031 | 0.149 ± 0.012 |
| Testes (L) | g | 0.119 ± 0.008 | - |
| Testes (R) | g | 0.117 ± 0.012 | - |
| Ovary (L) | g | - | 0.030 ± 0.010 |
| Ovary (R) | g | - | 0.032 ± 0.017 |
Rag2 Urinalysis Data
Male - sampled 05/08/03
| Animal ID# | 01 | 02 | 03 | 04 | 05 | 06 | 07 | 08 | 09 | 10 |
|---|---|---|---|---|---|---|---|---|---|---|
| Glucose (mg/dl) | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. |
| Blood | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. |
| pH | 6.0 | 6.0 | 6.0 | 6.0 | 6.0 | 6.0 | 6.0 | 6.0 | 6.0 | 7.5 |
| Protein (mg/dl) | 30+ | 100++ | 30+ | 100++ | 30+ | 2000++++ | 300+++ | 100++ | 30+ | Neg. |
| Specific Gravity | 1.030 | 1.030 | 1.030 | 1.030 | 1.020 | 1.030 | 1.030 | 1.030 | 1.030 | 1.010 |
Female - sampled 05/07/03
| Animal ID# | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 |
|---|---|---|---|---|---|---|---|---|---|---|
| Glucose (g/dl) | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. |
| Blood | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. | Neg. |
| pH | 5.0 | 5.0 | 6.0 | 6.0 | 6.0 | 6.0 | 6.0 | 6.0 | 6.0 | 6.0 |
| Protein (mg/dl) | Trace | Trace | Trace | 30+ | Trace | Trace | 30+ | 30+ | 30+ | Trace |
| Specific Gravity | 1.030 | 1.030 | 1.030 | 1.030 | 1.030 | 1.030 | 1.030 | 1.030 | 1.030 | 1.020 |
Animal Receipt and Maintenance
A quantity of twenty mice (ten males and ten females) was submitted for testing, strain identified as Line 601(M) (C.129S6(B6)-Rag2tm1Fwa N12), age 7 weeks. Mice were received at RBU 3, Taconic Biotechnology, Albany NY and acclimated for three days on irradiated NIH #31M rodent diet and sterilized water ad lib, sterile contact bedding (paper chip) and a 12:12 light:dark cycle. All animals appeared normal during this period and routine health surveillance of this colony detected no microbial pathogens. Mice were assigned study unique identification numbers (males 1 -10, females 11- 20).
Urinalysis
All mice were fasted in metabolic cages and an overnight urine sample was collected. Urine analysis was performed using Multistix 10 SG (Bayer). Strips were read and recorded as per the manufacturer's instructions.
Clinical Chemistry and Hematology
A terminal blood sample was taken from Carbon Dioxide - anesthetized mice via cardiac puncture. The collected blood was divided into two samples. One sample was treated with EDTA and stored at 4°C for hematological evaluation. Another sample was allowed to clot at 4°C for 30 minutes, and then centrifuged at 7000rpm for 10 minutes, and the serum was decanted and frozen at -80°C for clinical chemistry analysis. A slide smear was made from a single drop of whole blood. Frozen serum, chilled whole blood and slides were delivered to LabCorp (RTP, NC) for analysis. Unless otherwise indicated, serum chemistry data is generated from a Hitachi 717 automated analyzer and hematological data is generated from a Celldyne 3500. WBC differential counts are performed manually.
Necropsy and Organ Weights
All mice were euthanized and bodyweights were recorded. Representative tissues were collected, weighed, and immersion fixed in 10% Neutral Buffered Formalin. Tissues were delivered to the Taconic lab for histological preparation and evaluation. The pathologist’s summary and detailed histological descriptions follow.
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Rag2 - Model 601
Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions for Taconic Models, Products and Services and the following terms of use:
- Title to these Models and biological materials derived from them remains with Taconic.
- The Models will be used for research purposes only.
- The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless additional rights have been granted in writing by Taconic.
- The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
- Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.
Pricing - USD
Opportunist Free (OF) Health Standard
601 Female
601-F Genotype ko/ko
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $220.00 |
Specialized Inventory
| 601-RF Genotype ko/ko | Quantity 1 - 199 |
| Retired Female Breeder | $220.00 |
601 Male
601-M Genotype ko/ko
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $220.00 |
Specialized Inventory
| 601-RM Genotype ko/ko | Quantity 1 - 199 |
| Retired Male Breeder | $220.00 |
Pricing - EUR
Opportunist Free (OF) Health Standard
601 Female
601-F Genotype ko/ko
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €200,00 |
Specialized Inventory
| 601-RF Genotype ko/ko | Quantity 1 - 199 |
| Retired Female Breeder | €200,00 |
601 Male
601-M Genotype ko/ko
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €200,00 |
Specialized Inventory
| 601-RM Genotype ko/ko | Quantity 1 - 199 |
| Retired Male Breeder | €200,00 |
Pricing - USD Nonprofit
Opportunist Free (OF) Health Standard
601 Female
601-F Genotype ko/ko
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $220.00 |
Specialized Inventory
| 601-RF Genotype ko/ko | Quantity 1 - 199 |
| Retired Female Breeder | $220.00 |
601 Male
601-M Genotype ko/ko
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $220.00 |
Specialized Inventory
| 601-RM Genotype ko/ko | Quantity 1 - 199 |
| Retired Male Breeder | $220.00 |
Pricing - EUR Nonprofit
Opportunist Free (OF) Health Standard
601 Female
601-F Genotype ko/ko
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €200,00 |
Specialized Inventory
| 601-RF Genotype ko/ko | Quantity 1 - 199 |
| Retired Female Breeder | €200,00 |
601 Male
601-M Genotype ko/ko
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €200,00 |
Specialized Inventory
| 601-RM Genotype ko/ko | Quantity 1 - 199 |
| Retired Male Breeder | €200,00 |
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