About the webinar:
This webinar will present the workflow for CRISPR/Cas9-based custom genetically engineered mouse models, overviewing technical and programmatic considerations. This will include a brief history of the use of the technology and the progression to today’s applications in model generation and recommendations for use. We will also review the importance of mosaicism assessment and screening for off-target mutations when using CRISPR/Cas9 and how data-informed selection of gene-edited founders is advantageous to model generation projects and study programs that require them.
We will outline details on the concept behind the ExpressMODEL ® CRISPR Platform and our approach to using next generation sequencing for molecular characterization of founder that includes mosaicism determination and analysis of predicted potential off-target sites. We will present how we use NGS data to ensure quality of the model and to shorten the time to study results. We will include examples that highlight the cost and time-saving benefits of choosing ExpressMODEL®
In addition, we will show how we apply the ExpressMODEL® concept to two other model generation methodologies, embryonic stem cell targeting and random integration transgenesis.
Watch this webinar to learn about:
- The workflow for custom mouse models generated using CRISPR/Cas9
- How Taconic uses next generation sequencing to assess mosaicism in founder mice and to screen for potential off-target mutations
- How molecular characterization of founder mice and germline transmission by in vitro fertilization reduces the time to results for programs requiring mouse models made by CRISPR/Cas9
