Taconic Biosciences' Coronavirus (COVID-19) Toolkit

  • Commercial Models
  • COVID-19 Disease Models
  • Antibody Development and Production
  • Vaccine Research and Development
  • Scientific Services

COVID-19 is a serious public health threat across the globe (see Taconic's Response to COVID-19). Taconic Biosciences is proud to support preclinical research aimed at neutralizing this threat, from basic research through vaccine development. As part of this commitment, we are subsidizing distribution of specialty transgenics including humanized ACE2 mice. See below for more details and check back frequently as we anticipate making updates to the model list.

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Note that we list research relevance based on the existing large body of research on SARS (SARS-CoV) as well as emerging reports of preclinical SARS-CoV-2 studies.

Commercial Models

COVID-19 Disease Models

Human ACE2 Mouse Models

Model #Model NameRelevance to COVID-19
18222hACE2 AC70The SARS-CoV-2 spike protein binds to ACE2 on the surface of cells to facilitate entry.1,2 Mouse ACE2 is sufficiently different from human ACE2 to prevent infection by SARS-CoV-2, meaning that wild type mice cannot be infected using clinical virus isolates. Mice expressing human ACE2 may support infection by SARS-CoV-2 and thus be useful for studies of vaccines, antiviral drugs and disease pathogenesis. Taconic has obtained several lines of hACE2 transgenic mice from the lab of Chien-Te (Kent) Tseng at the University of Texas Medical Branch.3,4 These lines were originally developed to study SARS, but may prove useful for COVID-19 studies.
18225hACE2 AC22

 

Ace2 Knockout Mouse

Model #Model NameRelevance to COVID-19
TF3738Ace2 Knockout MouseAcute respiratory distress syndrome (ARDS) is a serious complication of COVID-19 and present in a large percentage of COVID-19 deaths. ACE2 is protective against ARDS. Binding of viral spike SARS protein to ACE2 in mice downregulates ACE2 expression. Loss of ACE2 expression is associated with severe lung failure. Ace2 knockout mice have been used in ARDS and SARS research.2,5,6

The Ace2 Knockout Mouse was generated by Lexicon Pharmaceuticals and is part of Taconic's Knockout Repository under model # TF3738.

This model is currently cryopreserved. Please contact us if you are interested in using this model for COVID-19 research.

Tmprss2 Knockout Mouse

Model #Model NameRelevance to COVID-19
TF3746Tmprss2 Knockout MouseTMPRSS2 is involved in SARS-CoV-2 entry into cells. Inhibition of this protein may constitute a treatment/prophylaxis mechanism. Tmprss2 knockout mice may be useful in studying COVID-19 disease pathogenesis.2,7

 

This model is currently cryopreserved. Please contact us if you are interested in using this model for COVID-19 research.

Humanized DPP4 and Dpp4 Knockout Mice

Model #Model NameRelevance to COVID-19
TBDHumanized DPP4 MouseDPP4 is the receptor for MERS-CoV, the virus which causes Middle Eastern Respiratory Syndrome (MERS). Modeling using information from crystal structures identified DPP4 as a potential co-receptor for SARS-CoV-2; DPP4 may help facilitate entry into cells upon binding by the viral spike protein.8 Although wild type mice are not permissive for MERS infection due to differences between mouse and human DPP4, transgenic mice which express human DPP4 can be infected with MERS-CoV.9,10,11 Humanized and knockout DPP4 mice may be useful in studying COVID-19 disease pathogenesis.
TF3257Dpp4 Knockout Mouse
10053Dpp4 Conditional Knockout

 

Taconic has access to a humanized DPP4 mouse from the laboratory of Chien-Te (Kent) Tseng at the University of Texas Medical Branch and is developing plans to scale up and distribute this model. Please register your interest:

Humanized APOE and Apoe Knockout Mice

Model #Model NameRelevance to COVID-19
1549APOE4The human apolipoprotein E4 allele may be associated with risk of more severe COVID-19 infection and clinical outcomes compared to the E3 allele.12 APOE is involved in cholesterol transport, and the E4 allele is associated with atherosclerosis and Alzheimer's disease. APOE also plays an important immunomodulatory role and is implicated in some viral infections13, and ApoE4 is associated with heightened susceptibility to inflammation compared to ApoE2 and ApoE3 in both mice and humans.14,15 Taconic offers mice which carry human APOE alleles. Line 1549 is homozygous for the E4 allele, line 1548 is homozygous for the E3 allele, and line 1547 is homozygous for the E2 allele. Line APOE is a knockout of murine Apoe and may be a useful control for the humanized models. Taconic can produce custom hybrids which carry one E3 and one E4 allele upon request.
1548APOE3
1547APOE2
APOEApoe

 

Stat1 Knockout Mouse

Model #Model NameRelevance to COVID-19
2045Stat1Stat1 knockout mice support SARS-CoV viral replication in the lungs and develop progressive lung disease including diffuse interstitial pneumonia with inflammation and systemic spread to other organs. May be useful to study disease pathogenesis and antiviral treatments.16,17,18,19

 

Inbred Mice

Model #Model NameRelevance to COVID-19
BALB

BALJBO		BALB/c

BALB/c Bom		Young inbred mice such as BALB/c support viral replication of SARS and may be useful for vaccine and antiviral studies even without supporting development of disease. They may also be useful in studying immune responses to infection.19

In contrast to young mice, 12 to 14 month old BALB/c mice develop clinical illness including patchy interstitial pneumonia following SARS infection and may be useful to model the age-related mortality increase seen in humans in COVID-19.20

Mouse-adapted SARS strains induce clinical illness in BALB/c mice, with similar disease characteristics as seen in humans.21 Mouse-adapted SARS-CoV-2 strains have now been reported, and inbred mice may be useful for studies of vaccines, antiviral drugs and disease pathogenesis. Mouse-adapted SARS-CoV-2 can infect and replicate in both young and aged BALB/c mice, resulting in decreased pulmonary function, mild to moderate pneumonia and inflammation.22,23
B6

B6JBOM		Black 6

B6JBom		Young inbred mice such as C57BL/6 support viral replication of SARS and may be useful for vaccine and antiviral studies even without supporting development of disease. They may also be useful in studying immune responses to infection.19

Acute respiratory distress syndrome (ARDS) is a serious complication of COVID-19 and present in a large percentage of COVID-19 deaths. C57BL/6 mice have been used in various models of acute lung injury.

Aged C57BL/6J may also be useful to study age-related mortality increases but with lower viremia compared to BALB/c.24

Mouse-adapted SARS-CoV-2 strains have now been reported, and inbred mice may be useful for studies of vaccines, antiviral drugs and disease pathogenesis.22,23
129S6129SVEYoung inbred mice such as 129S6 support viral replication of SARS and may be useful for vaccine and antiviral studies even without supporting development of disease. They may also be useful in studying immune responses to infection.19

The 129S6 inbred strain is the appropriate wild type control for use with Taconic's Stat1 Knockout mice (model #2045).

Aged 129S6 may also be useful to study age-related mortality increases but with lower viremia compared to BALB/c.24

Mouse-adapted SARS-CoV-2 strains have now been reported, and inbred mice may be useful for studies of vaccines, antiviral drugs and disease pathogenesis.22,23

Rodent temperature monitoring technology

Body temperature can be a useful clinical indicator in infectious disease mouse models, generally presenting as a drop in core body temperature.26 Although body temp data may be a useful readout for many types of studies, it is often not assessed due to measurement challenges. Implantable RFID temperature probes provide a remote tool for measuring mouse or rat body temperature following experimental infections. This provides several benefits, including: (1) the ability to monitor body temperature in real time to better quantify clinical disease course, (2) improved biosafety by maintaining animals in their cage, and (3) improved humane endpoints.

 

Taconic can implant RFID temperature microchips from Unified Information Devices (UID) into any mouse or rat line. Inquire for details. UID is offering a free microchip reader for qualifying purchases to support COVID-19 research.

Antibody Development and Production

Model #Model NameRelevance to COVID-19
BALB

BALJBO

SW

ICRSC		BALB/c

BALB/c Bom

Swiss Webster

ICR scid		Monoclonal and polyclonal antibodies to SARS-CoV-2 will be useful research reagents for a variety of applications. When developing a monoclonal antibody, Swiss Webster and BALB/c mice are often used for immunization and B cells harvested for fusion to myeloma cells (hybridoma). Once a monoclonal hybridoma of choice is identified, the cells may be scaled up for in vitro production or injected into mice such as BALB/c (for mouse hybridomas) or ICR scid (for mouse or xenogeneic hybridomas) for in vivo production.

Taconic BALB have been used to generate SARS-CoV-neutralizing antibodies.27

Vaccine Research and Development

Transgenic HLA Mice

Model #Model NameRelevance to COVID-19
9659

9660

9661

9662

9663

9664

4149		HLA-A2.1 (CB6F1)

HLA-A11 (CB6F1)

HLA-B7 (C;B6)

HLA-A1 (CB6F1)

HLA-A24 (CB6F1)

HLA-B44 (CB6F1)

Abb Knockout/Transgenic HLA-DR4		Rationally-designed vaccine development is complicated by differences between species in the antigens presented by major histocompatibility complex (MHC) proteins. MHC is called human leukocyte antigen (HLA) in humans. The human population carries a wide variety of HLA genes which can be divided into supertypes based on similar antigen binding. Mice which carry human HLA genes are useful in vaccine discovery and development because they better model the human response to vaccines. Taconic has a portfolio of six transgenic HLA class I models designed around supertypes which give coverage for 99+% of the human population as well as one HLA Class II model.

 

Humanized Immune System Mice

Model #Model NameRelevance to COVID-19
huPBMC-NOG

huPBMC-14957

HSCCB-NOG

HSCCB-13395		huPBMC-NOG

huPBMC-B2m-NOG

huNOG

huNOG-EXL		PBMC-engrafted NOG mice (model huPBMC-NOG) have been used to study SARS vaccine response. Humanized immune system mice may be useful for studies into human immune system response to infection and/or vaccine response.28

 

Scientific Services

Colony Management Solutions

During this difficult and overwhelming time as the COVID-19 situation continues to evolve, Taconic recognizes the need within the research community for stability and reliability. We are committed to providing you with the products and services you depend on.

Our colony management solutions can offer relief to labs and vivaria across the world that are faced with social distancing restrictions, staff reductions, or temporary closures. We are happy to provide our cryopreservation services at a highly competitive price to alleviate your worries and ensure the security of your valuable genetically modified models for the future. If you have questions about safeguarding your research animals, we suggest downloading our White Paper The Integral Role of Cryopreservation in Rodent Colony Management.

Model Generation Solutions

Additionally, we will continue to offer our world-renowned model generation and consultation services by leveraging the most comprehensive, fully licensed gene modification toolkit in the industry. As your lab work is likely slowing down, Taconic's PhD scientists can help you shift gears and bring to fruition a project that has been stuck on the back burner or design a model for your upcoming grant application. Let us do the work now, so you can focus on more immediate concerns for your future during these rapidly changing times.

Scientific Services

Colony Management Solutions

During this difficult and overwhelming time as the COVID-19 situation continues to evolve, Taconic recognizes the need within the research community for stability and reliability. We are committed to providing you with the products and services you depend on.

Our colony management solutions can offer relief to labs and vivaria across the world that are faced with social distancing restrictions, staff reductions, or temporary closures. We are happy to provide our cryopreservation services at a highly competitive price to alleviate your worries and ensure the security of your valuable genetically modified models for the future. If you have questions about safeguarding your research animals, we suggest downloading our White Paper The Integral Role of Cryopreservation in Rodent Colony Management.

Consult with a PhD scientist

Model Generation Solutions

Additionally, we will continue to offer our world-renowned model generation and consultation services by leveraging the most comprehensive, fully licensed gene modification toolkit in the industry. As your lab work is likely slowing down, Taconic's PhD scientists can help you shift gears and bring to fruition a project that has been stuck on the back burner or design a model for your upcoming grant application. Let us do the work now, so you can focus on more immediate concerns for your future during these rapidly changing times.

Consult with a PhD scientist

References:

1. Zhou, P.; Yang, X.-L.; Wang, X.-G.; Hu, B.; Zhang, L.; Zhang, W.; Si, H.-R.; Zhu, Y.; Li, B.; Huang, C.-L.; Chen, H.-D.; Chen, J.; Luo, Y.; Guo, H.; Jiang, R.-D.; Liu, M.-Q.; Chen, Y.; Shen, X.-R.; Wang, X.; Zheng, X.-S.; Zhao, K.; Chen, Q.-J.; Deng, F.; Liu, L.-L.; Yan, B.; Zhan, F.-X.; Wang, Y.-Y.; Xiao, G.-F.; Shi, Z.-L. A Pneumonia Outbreak Associated with a New Coronavirus of Probable Bat Origin. Nature. 2020, 579 (7798), 270-273.

2. Hoffmann, M.; Kleine-Weber, H.; Schroeder, S.; Krüger, N.; Herrler, T.; Erichsen, S.; Schiergens, T. S.; Herrler, G.; Wu, N.-H.; Nitsche, A.; Müller, M. A.; Drosten, C.; Pöhlmann, S. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell 2020.

3. Tseng, C.-T. K.; Huang, C.; Newman, P.; Wang, N.; Narayanan, K.; Watts, D. M.; Makino, S.; Packard, M. M.; Zaki, S. R.; Chan, T.-S.; Peters, C. J. Severe Acute Respiratory Syndrome Coronavirus Infection of Mice Transgenic for the Human Angiotensin-Converting Enzyme 2 Virus Receptor. Journal of Virology 2007, 81 (3), 1162-1173.

4. Yoshikawa, N.; Yoshikawa, T.; Hill, T.; Huang, C.; Watts, D. M.; Makino, S.; Milligan, G.; Chan, T.; Peters, C. J.; Tseng, C.-T. K. Differential Virological and Immunological Outcome of Severe Acute Respiratory Syndrome Coronavirus Infection in Susceptible and Resistant Transgenic Mice Expressing Human Angiotensin-Converting Enzyme 2. Journal of Virology 2009, 83 (11), 5451-5465.

5. Imai, Y.; Kuba, K.; Rao, S.; Huan, Y.; Guo, F.; Guan, B.; Yang, P.; Sarao, R.; Wada, T.; Leong-Poi, H.; Crackower, M. A.; Fukamizu, A.; Hui, C.-C.; Hein, L.; Uhlig, S.; Slutsky, A. S.; Jiang, C.; Penninger, J. M. Angiotensin-Converting Enzyme 2 Protects from Severe Acute Lung Failure. Nature 2005, 436 (7047), 112-116.

6. Kuba, K.; Imai, Y.; Rao, S.; Gao, H.; Guo, F.; Guan, B.; Huan, Y.; Yang, P.; Zhang, Y.; Deng, W.; Bao, L.; Zhang, B.; Liu, G.; Wang, Z.; Chappell, M.; Liu, Y.; Zheng, D.; Leibbrandt, A.; Wada, T.; Slutsky, A. S.; Liu, D.; Qin, C.; Jiang, C.; Penninger, J. M. A Crucial Role of Angiotensin Converting Enzyme 2 (ACE2) in SARS Coronavirus-Induced Lung Injury. Nature Medicine 2005, 11 (8), 875-879.

7. Iwata-Yoshikawa, N.; Okamura, T.; Shimizu, Y.; Hasegawa, H.; Takeda, M.; Nagata, N. TMPRSS2 Contributes to Virus Spread and Immunopathology in the Airways of Murine Models after Coronavirus Infection. Journal of Virology 2019, 93 (6).

8. Li, Y.; Zhang, Z.; Yang, L.; Lian, X.; Xie, Y.; Li, S.; Xin, S.; Cao, P.; Lu, J. The MERS-CoV Receptor DPP4 as a Candidate Binding Target of the SARS-CoV-2 Spike. iScience2020, 23 (6), 101160.

9. Agrawal, A. S.; Garron, T.; Tao, X.; Peng, B.-H.; Wakamiya, M.; Chan, T.-S.; Couch, R. B.; Tseng, C.-T. K. Generation of a Transgenic Mouse Model of Middle East Respiratory Syndrome Coronavirus Infection and Disease. Journal of Virology 2015, 89 (7), 3659-3670.

10. Li, K.; Wohlford-Lenane, C. L.; Channappanavar, R.; Park, J.-E.; Earnest, J. T.; Bair, T. B.; Bates, A. M.; Brogden, K. A.; Flaherty, H. A.; Gallagher, T.; Meyerholz, D. K.; Perlman, S.; Mccray, P. B. Mouse-Adapted MERS Coronavirus Causes Lethal Lung Disease in Human DPP4 Knockin Mice. Proceedings of the National Academy of Sciences 2017, 114 (15).

11. Fan, C.; Wu, X.; Liu, Q.; Li, Q.; Liu, S.; Lu, J.; Yang, Y.; Cao, Y.; Huang, W.; Liang, C.; Ying, T.; Jiang, S.; Wang, Y. A Human DPP4-Knockin Mouse's Susceptibility to Infection by Authentic and Pseudotyped MERS-CoV. Viruses 2018, 10 (9), 448.

12. Kuo, C.-L.; Pilling, L. C.; Atkins, J. L.; Masoli, J. A.; Delgado, J.; Kuchel, G. A.; Melzer, D. Apoe E4 Genotype Predicts Severe Covid-19 In The Uk Biobank Community Cohort. 2020.

13. Tudorache, I. F.; Trusca, V. G.; Gafencu, A. V. Apolipoprotein E - A Multifunctional Protein with Implications in Various Pathologies as a Result of Its Structural Features. Computational and Structural Biotechnology Journal 2017, 15, 359-365.

14. Rodriguez, G. A.; Tai, L. M.; Ladu, M.; Rebeck, G. Human APOE4 Increases Microglia Reactivity at Aβ Plaques in a Mouse Model of Aβ Deposition. Journal of Neuroinflammation2014, 11 (1), 111.

15. Gale, S.C.; Gao, L.; Mikacenic, C.; Coyle, S.M.; Rafaels, N.; Murray Dudenkov, T.; et al. APOepsilon4 is associated with enhanced in vivo innate immune responses in human subjects. The Journal of Allergy and Clinical Immunology 2014, 134 (1), 127-34.

16. Hogan, R. J.; Gao, G.; Rowe, T.; Bell, P.; Flieder, D.; Paragas, J.; Kobinger, G. P.; Wivel, N. A.; Crystal, R. G.; Boyer, J.; Feldmann, H.; Voss, T. G.; Wilson, J. M. Resolution of Primary Severe Acute Respiratory Syndrome-Associated Coronavirus Infection Requires Stat1. Journal of Virology 2004, 78 (20), 11416-11421.

17. Frieman, M. B.; Chen, J.; Morrison, T. E.; Whitmore, A.; Funkhouser, W.; Ward, J. M.; Lamirande, E. W.; Roberts, A.; Heise, M.; Subbarao, K.; Baric, R. S. SARS-CoV Pathogenesis Is Regulated by a STAT1 Dependent but a Type I, II and III Interferon Receptor Independent Mechanism. PLoS Pathogens 2010, 6 (4).

18. Graham, R. L.; Becker, M. M.; Eckerle, L. D.; Bolles, M.; Denison, M. R.; Baric, R. S. A Live, Impaired-Fidelity Coronavirus Vaccine Protects in an Aged, Immunocompromised Mouse Model of Lethal Disease. Nature Medicine 2012, 18 (12), 1820-1826.

19. Subbarao, K.; Roberts, A. Is There an Ideal Animal Model for SARS? Trends in Microbiology 2006, 14 (7), 299-303.

20. Roberts, A.; Paddock, C.; Vogel, L.; Butler, E.; Zaki, S.; Subbarao, K. Aged BALB/c Mice as a Model for Increased Severity of Severe Acute Respiratory Syndrome in Elderly Humans. Journal of Virology 2005, 79 (9), 5833-5838.

21. Day, C. W.; Baric, R.; Cai, S. X.; Frieman, M.; Kumaki, Y.; Morrey, J. D.; Smee, D. F.; Barnard, D. L. A New Mouse-Adapted Strain of SARS-CoV as a Lethal Model for Evaluating Antiviral Agents in Vitro and in Vivo. Virology 2009, 395 (2), 210-222.

22. Dinnon, K. H.; Leist, S. R.; Schäfer, A.; Edwards, C. E.; Martinez, D. R.; Montgomery, S. A.; West, A.; Yount, B. L.; Hou, Y. J.; Adams, L. E.; Gully, K. L.; Brown, A. J.; Huang, E.; Bryant, M. D.; Choong, I. C.; Glenn, J. S.; Gralinski, L. E.; Sheahan, T. P.; Baric, R. S. A Mouse-Adapted SARS-CoV-2 Model for the Evaluation of COVID-19 Medical Countermeasures. 2020.

23. Gu, H.; Chen, Q.; Yang, G.; He, L.; Fan, H.; Deng, Y.-Q.; Wang, Y.; Teng, Y.; Zhao, Z.; Cui, Y.; Li, Y.; Li, X.-F.; Li, J.; Zhang, N.; Yang, X.; Chen, S.; Zhao, G.; Wang, X.; Luo, D.; Wang, H.; Yang, X.; Li, Y.; Han, G.; He, Y.; Zhou, X.; Geng, S.; Sheng, X.; Jiang, S.; Sun, S.; Qin, C.-F.; Zhou, Y. Rapid Adaptation of SARS-CoV-2 in BALB/c Mice: Novel Mouse Model for Vaccine Efficacy. 2020.

24. Roberts, A.; Lamirande, E. W.; Vogel, L.; Jackson, J. P.; Paddock, C. D.; Guarner, J.; Zaki, S. R.; Sheahan, T.; Baric, R.; Subbarao, K. Animal Models and Vaccines for SARS-CoV Infection. Virus Research 2008, 133 (1), 20-32.

25. Feng, Z.; Wang, Y.; Qi, W. The Small Intestine, an Underestimated Site of SARS-CoV-2 Infection: From Red Queen Effect to Probiotics. 2020.

26. Trammell RA, Toth LA. Markers for predicting death as an outcome for mice used in infectious disease research. Comp Med. 2011 Dec;61(6):492-8.

27. Faber, M.; Lamirande, E. W.; Roberts, A.; Rice, A. B.; Koprowski, H.; Dietzschold, B.; Schnell, M. J. A Single Immunization with a Rhabdovirus-Based Vector Expressing Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) S Protein Results in the Production of High Levels of SARS-CoV-Neutralizing Antibodies. Journal of General Virology 2005, 86 (5), 1435-1440.

28. Okada, M.; Okuno, Y.; Hashimoto, S.; Kita, Y.; Kanamaru, N.; Nishida, Y.; Tsunai, Y.; Inoue, R.; Nakatani, H.; Fukamizu, R.; Namie, Y.; Yamada, J.; Takao, K.; Asai, R.; Asaki, R.; Kase, T.; Takemoto, Y.; Yoshida, S.; Peiris, J.; Chen, P.-J.; Yamamoto, N.; Nomura, T.; Ishida, I.; Morikawa, S.; Tashiro, M.; Sakatani, M. Development of Vaccines and Passive Immunotherapy against SARS Corona Virus Using SCID-PBL/Hu Mouse Models. Vaccine2007, 25 (16), 3038-3040.