NFκB-RE-luc

NFκB-RE-luc Random Transgenic Mouse Model
  • Cryopreserved
  • Mouse
  • Albino
  • Not available for direct purchase by CRO
Model No.NomenclatureGenotype
10499 BALB/c-Tg(Rela-luc)31XenInquire for genotype
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Overview

Nomenclature: BALB/c-Tg(Rela-luc)31Xen

  • Carries a transgene containing 6 NFκB-responsive elements (RE) from the CMVα (immediate early) promoter placed upstream of a basal SV40 promoter, and a modified firefly luciferase cDNA (Promega pGL3)
  • Basal expression of the reporter is observed in the whole body
  • The reporter is inducible by LPS and TNFα
  • The model provides for the rapid study of transcriptional regulation of the NFκB gene
  • Useful in studying sepsis, arthritis, inflammatory bowel disease, apoptosis, tumor development, NFκB gene regulation, and the treatment of inflammatory diseases and cancer
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Origin

The NFκB-RE-luc mouse was developed by Caliper Life Sciences. The model was created by microinjecting a transgene containing 6 NFκB-responsive elements (RE) from the CMVα (immediate early) promoter placed upstream of a basal SV40 promoter, and a modified firefly luciferase cDNA (Promega pGL3). This transgene was microinjected into BALB/cJ zygotes. The resultant mice from founder line 31 were bred to BALB/cJ mice. Taconic received stock from Caliper in 2010, and the line was embryo transfer derived. The line was maintained by mating mice which are wild type to those which are hemizygous for the luciferase transgene.

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