NOG-EXL (hGM-CSF/hIL-3 NOG)

NOG-EXL (hGM-CSF/hIL-3 NOG) Transgenic Immunodeficient Mouse
  • Live
  • Mouse
  • Male & Female Available
  • Albino
  • Available for direct purchase by CRO
Model No.NomenclatureGenotype
13395-FNOD.Cg-Prkdcscid Il2rgtm1Sug Tg(SV40/HTLV-IL3,CSF2)10-7Jic/JicTacsp/sp;ko/ko;tg/wt
13395-MNOD.Cg-Prkdcscid Il2rgtm1Sug Tg(SV40/HTLV-IL3,CSF2)10-7Jic/JicTacsp/sp;ko/y;tg/wt
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Overview

Nomenclature: NOD.Cg-Prkdcscid Il2rgtm1Sug Tg(SV40/HTLV-IL3,CSF2)10-7Jic/JicTac

The hGM-CSF/hIL-3 NOG is an immunodeficient CIEA NOG mouse altered to express human GM-CSF and IL-3 cytokines. The resulting transgenic mouse model supports the differentiation of human myeloid cell lineages and potentiates increased efficiency of hematopoietic stem cell and human immune system engraftment compared to the core NOG mouse.

The model was designed to express relatively low cytokine levels, which maintains the long-term stability of engraftment by preventing the exhaustion of the stem cell pool. This stability makes it a flexible platform for the generation of humanized mice.

  • Supports myeloid lineage engraftment through human IL-3 and GM-CSF cytokine expression
  • More efficient human hematopoietic stem cell (HSC) engraftment
  • Higher levels of myeloid cell differentiation following human HSC engraftment
  • Low cytokine expression and long-term HSC engraftment
Research applications include the generation of humanized mouse models, oncology, infectious disease, and regenerative medicine.

Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

What our customers say:

“Experimental Pharmacology & Oncology (EPO) GmbH uses 1st and 2nd generation NOG mice provided by Taconic for its preclinical oncology service. These mice are included in novel concepts for the development of personalized treatment options and especially suited for humanization strategies. We are very satisfied with the quality of mice, the reliability of shipment and the high level of scientific support. Further, we very much appreciate the competent and always friendly communication.”
Experimental Pharmacology & Oncology (EPO) GmbH

Availability

Available now*
*Please note that advanced order placement is encouraged for male mice. Ordering 4 or more weeks prior to the desired shipping date is recommended when requesting males older than 3-weeks of age.

Origin

The CIEA NOG mouse® was developed by Mamoru Ito of the Central Institute for Experimental Animals (CIEA) in Japan. The Prkdc scid mutation was identified by Mel Bosma of the Fox Chase Cancer Center in a C.B-17 congenic mouse population. This mutation was backcrossed onto the NOD/ShiJic strain at CIEA for at least eight generations. The Il2rg targeted mutation was developed by Dr. Kazuo Sugamura of Tohoku University by targeting the gene in ES cells derived from a 129 strain. Portions of exons 7 and 8 were replaced with a neo cassette. Targeted ES cells were injected into C57BL/6 blastocysts. Resultant chimeras were backcrossed onto the C57BL/6JJic background for at least eight generations. The CIEA NOG mouse® was developed by backcrossing the C57BL/6JJic-Il2rg line to the NOD/ShiJic-Prkdc scid line for a total of eight generations. The hGM-CSF/hIL-3 SCID mouse was developed in the laboratory of Dr. Mamoru Ito of the Central Institute for Experimental Animals (CIEA) in Japan. The linearized hIL-3 and hGM-CSF transgenes were co-injected into zygotes from BDF1 females x C57BL/6J scid. The transgenic SCID mouse was backcrossed more than 12 generations on to the CIEA NOG mouse® background. Taconic received stock in 2014, and the line was derived through embryo transfer.

Genetics

Species:Mouse
Strain Type:Congenic
Allele Type:Constitutive Knockout;Spontaneous Mutant;Random Transgenic;Genetically Humanized
Coat Color:Albino
Genetic Background:NOD

Guides & Publications

Initial Publication: 

  • Ito M, Hiramatsu H, Kobayashi K, Suzue K, Kawahata M, Hioki K, Ueyama Y, Koyanagi Y, Sugamura K, Tsuji K, Heike T, Nakahata T. (2002) NOD/SCID/γ mouse: an excellent recipient mouse model for engraftment of human cells. Blood 100(9):3175-3182.
  • Fukuchi Y, Miyakawa Y, Kobayashi K, Kuramochi T, Shimamura K, Tamaoki N, Nomura T, Ueyama Y, Ito M. (1998) Cytokine dependent growth of human TF-1 leukemic cell line in human GM-CSF and IL-3 producing transgenic SCID mice. Leuk Res 22(9):837-43.
  • Ito R, Takahashi T, Katano I, Kawai K, Kamisako T, Ogura T, Ida-Tanaka M, Suemizu H, Nunomura S, Ra C, Mori A, Aiso S, Ito M. (2013) Establishment of a human allergy model using human IL-3/GM-CSF-transgenic NOG mice. J Immunol.191(6):2890-9.

Applications & Therapeutic Areas

  • Cell and Tissue Humanized
  • Genetically Humanized
  • Immunology
  • Infectious Disease
  • Inflammation
  • Oncology & Immuno-Oncology
  • Vaccine Research

Transit, Housing & Welfare

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Packing Practices

Taconic standard practice is to recombine animals of different home cages and/or ages from a single model and sex during packing, except in specific cases where Taconic's animal welfare policy prohibits recombination due to aggression or other concerns.  When an order is fulfilled with animals from more than one week of birth, this standard practice results in animals from a range of birth weeks packed together in a single TTC. When an order is fulfilled with animals from genotyped models, this standard practice results in animals from different home cages packed together in a single TTC.

Customers who wish to keep animals from different weeks of birth separated should place orders with the special instruction "Divide and label by age."  Note that this special request can result in increased costs for additional Taconic Transit Cages, dividers and/or freight charges.

Taconic discourages other types of custom packing requests as they can have a negative impact on animal welfare. Learn more.

NOG Portfolio

mouse

NOG-EXL (hGM-CSF/hIL-3 NOG)

Nonprofit users (excluding users at nonprofit foundations which are affiliated with a for-profit entity): For internal research purposes, the CIEA NOG mouse® Conditions of Use for nonprofit users apply. If you wish to perform sponsored research or fee-for-service contract research using the CIEA NOG mouse®, please inquire for access conditions.

For-profit users and users at foundations which are affiliated with for-profit entities: The CIEA NOG mouse® Conditions of Use for for-profit users apply.

The CIEA NOG mouse® is produced and distributed under license rights to the following patents and trademarks:

  • Japanese Patent No. 3,753,321
  • US Patent No. 7,145,055; 5,464,764; 5,487,992; 5,627,059; 5,631,153; 5,789,215; 6,204,061; 6,653,113; 6,689,610
    EP Patent No. 1,338,198
  • Japanese Trademark Reg. No. 4,823,423
  • US Trademark Reg. No. 3,118,040
  • EU Trademark Reg. No. 3,736,758

Pricing - USD

Opportunist Free (OF) Health Standard

13395 Female

13395-F Genotype sp/sp;ko/ko;tg/wt

Age in WeeksQuantity 1 - 999
4 to 10$452.00

13395 Male

13395-M Genotype sp/sp;ko/y;tg/wt

Age in WeeksQuantity 1 - 999
4 to 10$389.00

Pricing - EUR

Opportunist Free (OF) Health Standard

13395 Female

13395-F Genotype sp/sp;ko/ko;tg/wt

Age in WeeksQuantity 1 - 999
4 to 10€410,00

13395 Male

13395-M Genotype sp/sp;ko/y;tg/wt

Age in WeeksQuantity 1 - 999
4 to 10€354,00

Pricing - USD Nonprofit

Opportunist Free (OF) Health Standard

13395 Female

13395-F Genotype sp/sp;ko/ko;tg/wt

Age in WeeksQuantity 1 - 999
4 to 10$269.00

13395 Male

13395-M Genotype sp/sp;ko/y;tg/wt

Age in WeeksQuantity 1 - 999
4 to 10$228.00

Pricing - EUR Nonprofit

Opportunist Free (OF) Health Standard

13395 Female

13395-F Genotype sp/sp;ko/ko;tg/wt

Age in WeeksQuantity 1 - 999
4 to 10€238,00

13395 Male

13395-M Genotype sp/sp;ko/y;tg/wt

Age in WeeksQuantity 1 - 999
4 to 10€181,00

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Webinar On-Demand

ON-DEMAND WEBINAR | Humanized Mice in Preclinical Immuno-Oncology - Part I

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ON-DEMAND PRESENTATION | Humanized Immune System (HIS) Mice: Breakthrough Models for Immuno-Oncology Drug Discovery

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Humanized Mouse Models for Drug Discovery: The NOG Portfolio | WHITE PAPER
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NOG-EXL mouse model from Taconic outperforms NSG-SGM3 in new study

Read about the new paper from scientists at the University of Pennsylvania and Taconic showing improved outcomes in glioblastoma research using the NOG-EXL mouse model.

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Get your Taconic Biosciences 2024 Pricing Guide today. Personalized, customizable, links to up-to-date health reports, and additional information on Taconic's products and services.

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