| Model No. | Nomenclature | Genotype |
|---|---|---|
| 11227 | C57BL/6NTac-Atm1.1Arte Tyrtm1Arte | Inquire for genotype |
B6 Albino A++
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Overview
Nomenclature: C57BL/6NTac-Atm1.1Arte Tyrtm1Arte
- A new and improved B6 albino strain based on the C57BL/6NTac substrain.
- Contains two mutations, a mutation in the Tyr gene to introduce albinism, and a reversion of the non-agouti (a) locus to the agouti locus (A)
- Using the Taconic B6 Albino as a blastocyst host and mating the subsequent chimeras to a standard B6 mouse permits detection of ES cell germline transmission by coat color and ensures that the G1 animal is identical to the B6 genome at all coat color loci
- The Taconic B6 albino is an ideal host for maintaining substrain specificity ES cells derived from the C57BL/6NTac substrain
- Can also be used as a genetic background for models in which a B6 background is needed with an albino coat, for example in imaging applications
- Available only from Taconic!
- Please note that chimeras generated using B6-derived ES cells and blastocysts from the B6 Albino A++ will be tri-colored. The agouti signalling peptide (produced in blastocyst-derived cells, which appear albino in the chimera) can produce a paracrine signalling effect in some cells derived from the ES cells. ES cell-derived hair patches can thus be either black or agouti. Both black and agouti patches should be counted for determination of chimerism percentages.
Origin
The B6 Albino A++ model was developed by Taconic. In C57BL/6 mice, intron 1 of the agouti locus contains a retrotransposon which is responsible for the non-agouti phenotype. In the Taconic B6, the region containing this retrotransposon was replaced with a FRT-flanked neomycin-puromycin resistance cassette in C57BL/6NTac-derived ES cells via gene targeting. The constitutive knock out allele was then generated via Flp-mediated removal of the selection cassette. The Tyr mutation is a constitutive knock in of a point mutation in which a G nucleotide located at position 369 in exon 1 has been replaced with C nucleotide, resulting in an amino acid substitution at position 103 (Cys>Ser). The F3 site flanked selection marker was removed by via Flp recombinase. Mice containing the agouti and tyrosinase mutations were intercrossed, and then the line was intracrossed to homozygosity.
This model is cryopreserved and available for recovery. Models can typically be recovered and delivered to customers within 14-16 weeks after order receipt. Purchase of this model includes perpetual use rights and a deliverable of four mutant animals at the Murine Pathogen Free™ health standard along with a genotyping protocol. For models which include a recombinase gene or multiple alleles, all alleles will be provided, but individual animals may not contain all mutant alleles.
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Genetics
Guides & Publications
Initial Publication:
Zevnik B, Uyttersprot NC, Perez AV, Bothe GW, Kern H, Kauselmann G. (2014) C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. PLoS One. 2014 Mar 5;9(3):e90570.
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Diet
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B6 Albino A++
Customers may purchase this model by signing the appropriate Terms of Sale (below) and providing a PO.
This model is sold under terms which grant perpetual use rights.
This model is sold under terms which grant perpetual use rights.
Pricing - USD
11227
| Item | Commercial | Nonprofit |
|---|---|---|
| Cryopreserved Model | $21,000.00 | $17,325.00 |
Cryopreserved models are invoiced upon shipment of recovered animals. Once orders are placed, the full purchase price will be applied if the order is canceled. For orders greater than 4 animals, please contact Taconic for options.
Fees for Taconic Transit Cages™ and freight are in addition to the price above.
Pricing - EUR
11227
| Item | Commercial | Nonprofit |
|---|---|---|
| Cryopreserved Model | €17.850,00 | €15.855,00 |
Cryopreserved models are invoiced upon shipment of recovered animals. Once orders are placed, the full purchase price will be applied if the order is canceled. For orders greater than 4 animals, please contact Taconic for options.
Fees for Taconic Transit Cages™ and freight are in addition to the price above.
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