| Model No. | Nomenclature | Genotype |
|---|---|---|
| 12108 | NTac:SD-Tg(TH-EGFP)7Xen | tg/wt |
hTH-GFP Rat (Autosomal Insertion)
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Overview
Nomenclature: NTac:SD-Tg(TH-EGFP)7Xen
- Carries an EGFP transgene driven by the human Tyrosine Hydroxylase (hTH) promoter.
- The hTH-GFP transgene is NOT located on the X chromosome. This is a key difference from model 12141 (X-linked transgene). Additionally, model 12108 exhibits ectopic GFP expression in olfactory bulb whereas the GFP expression in model 12141 (the X-linked version) has more specificity for TH neurons.
- High level EGFP expression observed in dopaminergic neurons in brain structures such as, substantia nigra, ventral tegmental area, striatum, olfactory bulb, and hypothalamus, with minimal ectopic expression elsewhere in the brain.
- Dopamine neurons are susceptible to damage/loss in Parkinson's disease and therefore this rat can be used as a tool to study damage/loss of dopamine neurons, e.g., after MPTP or 6-OHDA treatment.
- Useful for in vivo anatomical visualization and micro-dissection of rat midbrain structures and axonal projections. High EGFP expression permits fluorescence imaging of brain slices.
- Useful for FACS purification and in vitro culture of dopamine neurons for studies of disease pathogenesis in culture. Since EGFP is more easily detected than TH immunostaining at early developmental stages, this rat may be particularly useful for early embryonic studies of the development of dopamine neurons.
- Since EGFP is more easily detected than TH immunostaining at early developmental stages, this rat may be particularly useful for early embryonic studies of the development of dopamine neurons.
- Homozygous rats were not identified from a carrier x carrier mating format suggesting the model is homozygous lethal. Further analysis will be carried out and our website will be updated once more information is available. Carrier x WT mating format is currently recommended for breeding.
Availability
As of April 1, 2018, The Michael J. Fox Foundation for Parkinson’s Research (MJFF) has instituted a new ordering policy for this model in an effort to prevent delays in order fulfillment, ensure models are shipped at the desired age, and maintain affordable pricing for all researchers accessing MJFF models. To achieve these goals, the new MJFF policy dictates that no one group can deplete the MJFF-sponsored colony. As such, orders will be limited to a total quantity of approximately 15-20 per researcher per month. Average lead time and age range may vary based on quantity and sex requested. Researchers requiring orders above this amount should establish a dedicated colony for their own use, either by breeding in-house or contracting with Taconic. For questions or concerns, please email tools@michaeljfox.org.Origin
Source: Model Sponsor: The Michael J. Fox Foundation
This model is cryopreserved and available for recovery. Models can typically be recovered and delivered to customers within 12 weeks after order receipt. Purchase of this model includes perpetual use rights and a deliverable of four mutant animals at the Murine Pathogen Free™ health standard along with a genotyping protocol. For models which include a recombinase gene or multiple alleles, all alleles will be provided, but individual animals may not contain all mutant alleles.
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Genetics
Guides & Publications
Initial Publication: Iacovitti L, Wei X, Cai J, Kostuk EW, Lin R, et al. (2014) The hTH-GFP Reporter Rat Model for the Study of Parkinson’s Disease. PLoS ONE 9(12): e113151. doi:10.1371/journal.pone.011315.
Applications & Therapeutic Areas
- Neuroscience
- "Parkinsons Disease"
- Reporter Lines
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Licensing
The hTH-GFP Rat Model may be purchased by for-profit or non-profit users under the following label license (no signatures required).
LABEL LICENSE AGREEMENT FOR hTH-GFP RAT
The hTH-GFP Rat Model (“Model”) is produced and distributed under rights to patents and/or intellectual property owned by or licensed to the Michael J. Fox Foundation (“MJFF”) and Taconic. Taconic has rights to distribute the Model on behalf of MJFF. MJFF maintains ownership of the Model and permits the sale of such model to each purchaser that agrees to the Terms and Conditions of the Sale and following terms of use:
- Title to the Model, any progeny, and biological materials derived from the foregoing remains with MJFF.
- The Model may be bred or crossbred by purchaser
- Purchaser shall inform MJFF of any crossbred animal generated (“Modified Progeny”), shall at MJFF's election deposit breeding pairs of each Modified Progeny with Taconic or another designee of MJFF and shall permit Taconic or another designee of MJFF to distribute the Modified Progeny on behalf of MJFF to non-profit institutions or for-profit institutions without any compensation to purchaser on terms similar to the terms and conditions by which purchaser received the Model. Purchaser assumes all liability for any claim or demand that Modified Progeny created by purchaser infringes on the intellectual property rights of any third party.
- The Model, progeny, or Modified Progeny may be used for research purposes only.
- The Model, any progeny, Modified Progeny, or biological materials will not be used for commercial purposes.
- The Model, any progeny, Modified Progeny, and biological materials derived from them will not be distributed to any third party.
- Purchaser assumes all risk from using the Model, any progeny, Modified Progeny, and biological materials.
- To the extent permissible by law, purchaser indemnifies, defends, and holds harmless MJFF from any claim or demand that purchaser's use or transfer of the Model, any progeny, Modified Progeny, or biological materials has caused harm, damage, or liability.
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