| Model No. | Nomenclature | Genotype |
|---|---|---|
| 4111-F | C57BL/6NTac.Cg-Rag2tm1Fwa Il2rgtm1Wjl | ko/ko;ko/ko |
| 4111-M | C57BL/6NTac.Cg-Rag2tm1Fwa Il2rgtm1Wjl | ko/ko;ko/y |
Rag2/Il2rg Double Knockout
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Overview
Nomenclature: C57BL/6NTac.Cg-Rag2tm1Fwa Il2rgtm1Wjl
- Contains a disruption of the recombination activating gene 2 (Rag2).
- Il2rg deletion ablates functional receptors for many cytokines including IL-2, IL-4, IL-7, IL-9, and IL-15.
- Mice which lack Rag2 and Il2rg do not develop functional T, B or NK cells.
- Analysis of spleen cells showed no detectable NK1.1+ cells, B220+ B cells or Thy1+ T cells. A small number of the 1-3 million cells obtained were CD45 low and probably are cells in the macrophage/monocyte lineage.
- Useful for transplantation of cells otherwise rejected by NK cells in less immunodeficient models.
- Useful in combination with wild type C57BL/6NTac mice and Rag2 knockout mice on the C57BL/6NTac background for adoptive transfer and mechanistic experiments to study the role of different immune cells.
Origin
The Rag2 knockout was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells. The Il2rg knockout was made by Xiqing Cao and Warren Leonard at the National Heart, Lung, and Blood Institute by targeting the Il2rg gene in 129S4/SvJae-derived J1 ES cells. Il2rg knockout founders were backcrossed 8 generations to C57BL/6J. Il2rg knockout mice were crossed to Rag2 knockouts on C57BL/10AiTac and maintained on a mixed B6;B10 background. The line was further backcrossed to C57BL/6NTac. SNP testing shows >99% C57BL/6 background genome.
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