| Model No. | Nomenclature | Genotype |
|---|---|---|
| 4111-F | C57BL/6NTac.Cg-Rag2tm1Fwa Il2rgtm1Wjl | ko/ko;ko/ko |
| 4111-M | C57BL/6NTac.Cg-Rag2tm1Fwa Il2rgtm1Wjl | ko/ko;ko/y |
Rag2/Il2rg Double Knockout
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Overview
Nomenclature: C57BL/6NTac.Cg-Rag2tm1Fwa Il2rgtm1Wjl
- Contains a disruption of the recombination activating gene 2 (Rag2).
- Il2rg deletion ablates functional receptors for many cytokines including IL-2, IL-4, IL-7, IL-9, and IL-15.
- Mice which lack Rag2 and Il2rg do not develop functional T, B or NK cells.
- Analysis of spleen cells showed no detectable NK1.1+ cells, B220+ B cells or Thy1+ T cells. A small number of the 1-3 million cells obtained were CD45 low and probably are cells in the macrophage/monocyte lineage.
- Useful for transplantation of cells otherwise rejected by NK cells in less immunodeficient models.
- Useful in combination with wild type C57BL/6NTac mice and Rag2 knockout mice on the C57BL/6NTac background for adoptive transfer and mechanistic experiments to study the role of different immune cells.
Origin
The Rag2 knockout was developed in the laboratory of Frederick W. Alt at Columbia University. The model was created by targeting the Rag2 gene in CCE ES cells. The Il2rg knockout was made by Xiqing Cao and Warren Leonard at the National Heart, Lung, and Blood Institute by targeting the Il2rg gene in 129S4/SvJae-derived J1 ES cells. Il2rg knockout founders were backcrossed 8 generations to C57BL/6J. Il2rg knockout mice were crossed to Rag2 knockouts on C57BL/10AiTac and maintained on a mixed B6;B10 background. The line was further backcrossed to C57BL/6NTac. SNP testing shows >99% C57BL/6 background genome.
Genetics
Guides & Publications
Initial Publication:
- Garcia S, DiSanto J, Stockinger B. Following the development of a CD4 T cell response in vivo: from activation to memory formation. Immunity 1999 Aug;11(2):163-71.
- Greenberg P, Riddell S. Deficient cellular immunity--finding and fixing the defects. Science. 1999 Jul 23;285(5427):546-51. Review.
- Cao X, Shores EW, Hu-Li J, Anver MR, Kelsall BL, Russell SM, Drago J, Noguchi M, Grinberg A, Bloom ET, et al. Defective lymphoid development in mice lacking expression of the common cytokine receptor gamma chain; Immunity, 1995 Mar;2(3):223-38.
- Shinkai Y, Rathbun G, Lam KP, Oltz EM, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall AM, and Alt FW. RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangemen; Cell, 1992 Mar 6;68(5):855-67.
Applications & Therapeutic Areas
- Cell and Tissue Humanized
- Immunology
- Inflammation
- Oncology & Immuno-Oncology
Transit, Housing & Welfare
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Packing Practices
Taconic standard practice is to recombine animals of different home cages and/or ages from a single model and sex during packing, except in specific cases where Taconic's animal welfare policy prohibits recombination due to aggression or other concerns. When an order is fulfilled with animals from more than one week of birth, this standard practice results in animals from a range of birth weeks packed together in a single TTC. When an order is fulfilled with animals from genotyped models, this standard practice results in animals from different home cages packed together in a single TTC.
Customers who wish to keep animals from different weeks of birth separated should place orders with the special instruction "Divide and label by age." Note that this special request can result in increased costs for additional Taconic Transit Cages, dividers and/or freight charges.
Taconic discourages other types of custom packing requests as they can have a negative impact on animal welfare. Learn more.
Diet
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Rag2/Il2rg Double Knockout
Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions for Taconic Models, Products and Services and the following terms of use:
- Title to these Models and biological materials derived from them remains with Taconic.
- The Models will be used for research purposes only.
- The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless additional rights have been granted in writing by Taconic.
- The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
- Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.
Pricing - USD
Opportunist Free (OF) Health Standard
4111 Female
4111-F Genotype ko/ko;ko/ko
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $216.00 |
4111 Male
4111-M Genotype ko/ko;ko/y
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $216.00 |
Pricing - EUR
Opportunist Free (OF) Health Standard
4111 Female
4111-F Genotype ko/ko;ko/ko
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €197,00 |
4111 Male
4111-M Genotype ko/ko;ko/y
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €197,00 |
Pricing - USD Nonprofit
Opportunist Free (OF) Health Standard
4111 Female
4111-F Genotype ko/ko;ko/ko
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $202.00 |
4111 Male
4111-M Genotype ko/ko;ko/y
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $202.00 |
Pricing - EUR Nonprofit
Opportunist Free (OF) Health Standard
4111 Female
4111-F Genotype ko/ko;ko/ko
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €184,00 |
4111 Male
4111-M Genotype ko/ko;ko/y
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €184,00 |
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