| Model No. | Nomenclature | Genotype |
|---|---|---|
| 8907-F | B6NTac.C-Tg(HLA-A*1101/H2-Kb)A11.01 | tg/wt |
| 8907-M | B6NTac.C-Tg(HLA-A*1101/H2-Kb)A11.01 | tg/wt |
HLA-A11 (C57BL/6)
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Overview
Nomenclature: B6NTac.C-Tg(HLA-A*1101/H2-Kb)A11.01
- Carries a transgene consisting of fragments of the human HLA-A*1101 gene and mouse H2-Kb gene which encodes a chimeric class I molecule consisting of the human HLA-A11 leader, α1 and α2 domains ligated to the murine α3, transmembrane and cytoplasmic H2-Kb domains.
- Expresses the chimeric HLA-A11 class I molecule on the surface of T and B cells.
- Represents the human HLA-A3 supertype.
- Useful for infectious disease research, vaccine development and testing, safety and immunogenicity testing as well as research directed towards oncology, autoimmune disorders, and syngeneic tumor studies utilizing C57BL/6-derived tumors.
- Permits identification of epitopes restricted to the HLA-A11 supertype.
Origin
The HLA-A11 model was generated by Pharmexa-Epimmune. The model was created by microinjecting a chimeric transgene combining a fragment of the cDNA clone of the human HLA-A*1101 gene that included leader sequence, α1 and α2 domains ligated to a fragment of the murine H2-Kb gene containing the α3, transmembrane and cytoplasmic domains. This transgene was microinjected into CxB6 zygotes. The resultant mice from Founder Line A11.01 were backcrossed six generations to C57BL/6J. Taconic received stock from Pharmexa-Epimmune in 2008. The line was embryo transfer derived, backcrossed to C57BL/6NTac for a total of 11 generationsand then intracrossed to homozygosity. In the production colony, wild type C57BL/6NTac animals are bred to homozygous males to produce hemizygous pups on the C57BL/6NTac background. In addition, another production colony is breeding BALB/cAnNTac females with homozygous males on the C57BL/6NTac background to produce hemizygous CB6F1 pups, which are sold under model #9660.
Genetics
Guides & Publications
Initial Publication:
Alexander J, Oseroff C, Sidney J, Wentworth P, Keogh E, Hermanson G, Chisari FV, Kubo RT, Grey HM, Sette A. (1997) Derivation of HLA-A11/Kb transgenic mice: functional CTL repertoire and recognition of human A11-restricted CTL epitopes. J Immunol 159(10):4753-61. Erratum in: J Immunol 1999 162(5):3104.
Applications & Therapeutic Areas
- Autoimmune Disease
- Immunology
- Infectious Disease
- Oncology & Immuno-Oncology
- Safety Assessment
- Vaccine Research
- Inflammation
Transit, Housing & Welfare
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Packing Practices
Taconic standard practice is to recombine animals of different home cages and/or ages from a single model and sex during packing, except in specific cases where Taconic's animal welfare policy prohibits recombination due to aggression or other concerns. When an order is fulfilled with animals from more than one week of birth, this standard practice results in animals from a range of birth weeks packed together in a single TTC. When an order is fulfilled with animals from genotyped models, this standard practice results in animals from different home cages packed together in a single TTC.
Customers who wish to keep animals from different weeks of birth separated should place orders with the special instruction "Divide and label by age." Note that this special request can result in increased costs for additional Taconic Transit Cages, dividers and/or freight charges.
Taconic discourages other types of custom packing requests as they can have a negative impact on animal welfare. Learn more.
Diet
Data
HLA expression on spleen cells in HLA-A11 mice on two genetic backgrounds
Figure 1: Expression of HLA-A11 in model #s 8907 and 9660 was measured on spleen cells by flow cytometry using a pan-HLA-ABC antibody. Significant expression of HLA-A11 is seen in both models compared to a wild type C57BL/6 control. Expression levels are somewhat lower in hemizygous mice of model #9660 (on a CB6F1 background) compared to homozygous or hemizygous mice of model #8907 (on a C57BL/6 background), but are sufficient for experimental performance as detailed in many published references. Specific functional relevance of expression differences between models is unclear, and the choice of model for a particular experiment should include not just expression levels but also the role of Th1 vs Th2 immune response, whether a congenic background is required for use in syngeneic engraftment or adoptive cell transfer experiments and other factors.
HLA expression histogram for spleen cells in HLA-A11 mice on two genetic backgrounds
Figure 2: Histograms for HLA-A11 expression on spleen cells in representative animals, as measured using a pan-HLA-ABC antibody. Grey = wild type C57BL/6 control. Blue = three model #8907 hemizygous female mice (C57BL/6 background). Red = three model #9660 hemizygous female mice (CB6F1 background).
- Licensing
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HLA-A11 (C57BL/6)
Conditions of Use for Taconic Transgenic Models™
Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions for Taconic Models, Products and Services and the following terms of use:
- Title to these Models and biological materials derived from them remains with Taconic.
- The Models will be used for research purposes only.
- The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless additional rights have been granted in writing by Taconic.
- The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
- Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.
Pricing - USD
Murine Pathogen Free (MPF) Health Standard
8907 Female
8907-F Genotype tg/wt
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $425.00 |
8907 Male
8907-M Genotype tg/wt
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $425.00 |
Pricing - EUR
Murine Pathogen Free (MPF) Health Standard
8907 Female
8907-F Genotype tg/wt
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €387,00 |
8907 Male
8907-M Genotype tg/wt
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €387,00 |
Pricing - USD Nonprofit
Murine Pathogen Free (MPF) Health Standard
8907 Female
8907-F Genotype tg/wt
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $250.00 |
8907 Male
8907-M Genotype tg/wt
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $250.00 |
Pricing - EUR Nonprofit
Murine Pathogen Free (MPF) Health Standard
8907 Female
8907-F Genotype tg/wt
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €228,00 |
8907 Male
8907-M Genotype tg/wt
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €228,00 |
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