Nomenclature: C.Cg-Igh-J^tm1Dhu

• B cell deficient mice are useful for basic mechanistic studies of immunologic mechanisms as well as host mice in syngeneic tumor studies using immunogenic test articles.
• Carries a deletion of the endogenous murine J segments of the Ig heavy chain locus
• In homozygotes, all four JH gene segments are absent, resulting in cells that cannot produce a complete, recombined version of the variable region of the heavy chain
• Have no detectable IgM or IgG in the sera
• A low level (about 1% of normal) of rearrangement of the light chain kappa gene family is detected in total bone marrow
• Cells of the B lineage are drastically altered both in developmental progression and in cell quantity
• Contain no mature (immunoglobulin-bearing) B-lymphocytes in the spleen, bone marrow, lymph nodes, peripheral blood or peritoneum
• T-lymphocyte development appears to proceed normally, based on surface phenotype and quantity of cells in the spleen; splenic lymphocytes are enriched for T cells due to the B cell deficit
• Provides a null background useful for gene replacement experimentation in normal immune response and autoimmune diseases
• Useful for studying non-B cell activity in pathogen-induced disease and mechanisms of antibody gene assembly and expression
• For use in syngeneic experiments, the Jh mouse is 96% congenic to the BALB/c background and may successfully engraft some cell lines of BALB/c origin
• Model 17758 is a similar model on the C57BL/6NTac background which may be used for syngeneic tumor experiments using cell lines derived from B6.
• Tumor growth kinetics may vary in immunodeficient mice compared to wild type mice of the same strain. A pilot study to evaluate tumor growth is best practice.

See the Related Taconic Biosciences Insight:

• Antibody-deficient mice overcome obstacles to efficacy testing of biologics

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