| Model No. | Nomenclature | Genotype |
|---|---|---|
| 12524-F | C57BL/6NTac-Gt(ROSA)26Sortm16(cre)Arte | ki/wt |
| 12524-M | C57BL/6NTac-Gt(ROSA)26Sortm16(cre)Arte | ki/wt |
Cre Deleter
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- Overview
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- Diet
Overview
Nomenclature: C57BL/6NTac-Gt(ROSA)26Sortm16(cre)Arte
- Ubiquitously active general Cre deleter.
- This transgene is suspected to be homozygous lethal.
- Maternal expression of Cre often leads to deletion of loxP flanked target genes in Cre-negative embryos.
- This model replaces model 6467. Model 12524 is fully congenic on C57BL/6NTac.
Origin
The Cre Deleter mouse was developed by Artemis Pharmaceuticals (now Taconic). The mutant was created by a targeted mutation knock in of Cre into the Gt(ROSA)26 locus. Cre is expressed under the control of the Gt(ROSA)26Sor gene. Additional sequence elements are adenovirus major late transcript splice acceptor sequence from the intron 1/exon2 boundary situated 5´ and FLP recombinase target (FRT) sequences (Saccharomyces cerevisiae) situated 3´ of Cre. Targeting occurred in C57BL/6N ES cells. The colony is maintained by breeding heterozygotes to wild type C57BL/6NTac mice.
Genetics
Guides & Publications
Initial Publication:
Otto C, Fuchs I, Kauselmann G, Kern H, Zevnik B, Andreasen P, Schwarz G, Altmann H, Klewer M, Schoor M, Vonk R, Fritzemeier KH. (2009) GPR30 does not mediate estrogenic responses in reproductive organs in mice. Biol Reprod 80(1):34-41.
Applications & Therapeutic Areas
- Recombinase-mediated Gene Deletion
- Transgenic Model Generation Support
Transit, Housing & Welfare
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Packing Practices
Taconic standard practice is to recombine animals of different home cages and/or ages from a single model and sex during packing, except in specific cases where Taconic's animal welfare policy prohibits recombination due to aggression or other concerns. When an order is fulfilled with animals from more than one week of birth, this standard practice results in animals from a range of birth weeks packed together in a single TTC. When an order is fulfilled with animals from genotyped models, this standard practice results in animals from different home cages packed together in a single TTC.
Customers who wish to keep animals from different weeks of birth separated should place orders with the special instruction "Divide and label by age." Note that this special request can result in increased costs for additional Taconic Transit Cages, dividers and/or freight charges.
Taconic discourages other types of custom packing requests as they can have a negative impact on animal welfare. Learn more.
Diet
- Licensing
- Pricing - USD
- Pricing - EUR
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Licensing
Access to this model requires execution of a Cre Deleter Crossbreeding Agreement. There are two types of agreements, a one-time license which permits a single crossbreeding event to a specific mouse line or a broad license which permits crossbreeding to an unlimited number of lines during the term.
The fees for a Cre Deleter Crossbreeding Agreement that permits crossing of the Cre Deleter to ONE (1) specified floxed line is:
The fees for a Cre Deleter Crossbreeding Agreement that permits crossing of the Cre Deleter to ONE (1) specified floxed line is:
- $650/€575 per line for non-profit users
- $1,700/€1.530 per line for for-profit users
- $4,725/€4.300 per year for non-profit users
- $12,600/€11.470 per year for for-profit users
Pricing - USD
Opportunist Free (OF) Health Standard
12524 Female
12524-F Genotype ki/wt
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $472.00 |
12524 Male
12524-M Genotype ki/wt
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $472.00 |
Pricing - EUR
Opportunist Free (OF) Health Standard
12524 Female
12524-F Genotype ki/wt
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €414,00 |
12524 Male
12524-M Genotype ki/wt
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €414,00 |
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