| Model No. | Nomenclature | Genotype |
|---|---|---|
| 12141 | NTac:SD-Tg(TH-EGFP)24Xen | Inquire for genotype |
hTH-GFP Rat (X-linked)
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Overview
Nomenclature: NTac:SD-Tg(TH-EGFP)24Xen
- Carries an EGFP transgene driven by the human Tyrosine Hydroxylase (hTH) promoter.
- High level specific EGFP expression observed in dopaminergic neurons in brain structures such as, substantia nigra, ventral tegmental area, striatum, olfactory bulb, and hypothalamus, with minimal ectopic expression elsewhere in the brain.
- Dopamine neurons are susceptible to damage/loss in Parkinson's disease and therefore this rat can be used as a tool to study damage/loss of dopamine neurons, e.g., after MPTP or 6-OHDA treatment.
- Useful for in vivo anatomical visualization and micro-dissection of rat midbrain structures and axonal projections. High EGFP expression permits fluorescence imaging of brain slices.
- Useful for FACS purification and in vitro culture of dopamine neurons for studies of disease pathogenesis in culture.
- Since EGFP is more easily detected than TH immunostaining at early developmental stages, this rat may be particularly useful for early embryonic studies of the development of dopamine neurons.
- The hTH-GFP transgene is located on the X chromosome. Thus a mating format of hemizygous female by SD male is used. Other mating formats are not recommended.
Origin
The hTH-GFP rat was developed in collaboration between The Michael J. Fox Foundation, Thomas Jefferson University, and Taconic. DNA fragments including 10.794 kb of the distal hTH promoter and 1.168 kb of proximal hTH promoter were ligated and placed upstream of the GFP reporter gene to create the hTH-GFP construct. The model was created through pronuclear injection of the hTH-GFP construct into NTac:SD zygotes. Founder line 12141 was bred to NTac:SD. The colony is maintained by mating transgenic females with SD:NTac males.
Source: Model Sponsor: The Michael J. Fox Foundation
This model is cryopreserved and available for recovery. Models can typically be recovered and delivered to customers within 12 weeks after order receipt. Purchase of this model includes perpetual use rights and a deliverable of four mutant animals at the Murine Pathogen Free™ health standard along with a genotyping protocol. For models which include a recombinase gene or multiple alleles, all alleles will be provided, but individual animals may not contain all mutant alleles.
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Genetics
Guides & Publications
Initial Publication:
Iacovitti L, Wei X, Cai J, Kostuk EW, Lin R, Gorodinsky A, Roman P, Kusek G, Das SS, Dufour A, Dave KD. (2013) The hTH-GFP Rat: A Novel Model for the Study of Parkinson's Disease; poster 329.12/I11 presented at the society for Neuroscience Annual Meeting 2013, San Diego, CA.
Applications & Therapeutic Areas
- Neuroscience
- "Parkinsons Disease"
- Reporter Lines
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Licensing
The hTH-GFP Rat Model may be purchased by for-profit or non-profit users under the following label license (no signatures required).
LABEL LICENSE AGREEMENT FOR hTH-GFP RAT
The hTH-GFP Rat Model (“Model”) is produced and distributed under rights to patents and/or intellectual property owned by or licensed to the Michael J. Fox Foundation (“MJFF”) and Taconic. Taconic has rights to distribute the Model on behalf of MJFF. MJFF maintains ownership of the Model and permits the sale of such model to each purchaser that agrees to the Terms and Conditions of the Sale and following terms of use:
- Title to the Model, any progeny, and biological materials derived from the foregoing remains with MJFF.
- The Model may be bred or crossbred by purchaser
- Purchaser shall inform MJFF of any crossbred animal generated (“Modified Progeny”), shall at MJFF's election deposit breeding pairs of each Modified Progeny with Taconic or another designee of MJFF and shall permit Taconic or another designee of MJFF to distribute the Modified Progeny on behalf of MJFF to non-profit institutions or for-profit institutions without any compensation to purchaser on terms similar to the terms and conditions by which purchaser received the Model. Purchaser assumes all liability for any claim or demand that Modified Progeny created by purchaser infringes on the intellectual property rights of any third party.
- The Model, progeny, or Modified Progeny may be used for research purposes only.
- The Model, any progeny, Modified Progeny, or biological materials will not be used for commercial purposes.
- The Model, any progeny, Modified Progeny, and biological materials derived from them will not be distributed to any third party.
- Purchaser assumes all risk from using the Model, any progeny, Modified Progeny, and biological materials.
- To the extent permissible by law, purchaser indemnifies, defends, and holds harmless MJFF from any claim or demand that purchaser's use or transfer of the Model, any progeny, Modified Progeny, or biological materials has caused harm, damage, or liability.
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