HLA-B44 (C57BL/6)

HLA-B44 (C57BL/6)
  • Live
  • Mouse
  • Male & Female Available
  • Black
  • Available for direct purchase by CRO
  • Taconic Transgenic Model
Model No.NomenclatureGenotype
8911-FC57BL/6NTac-Tg(HLA-B*4002/H2-Kb)B44.01/NTactg/wt
8911-MC57BL/6NTac-Tg(HLA-B*4002/H2-Kb)B44.01/NTactg/wt
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Overview

Nomenclature: C57BL/6NTac-Tg(HLA-B*4002/H2-Kb)B44.01/NTac

  • Carries a transgene consisting of fragments of the human HLA-B*4002 gene and mouse H2-Kb gene which encodes a chimeric class I molecule consisting of the human HLA-B44 leader, α1 and α2 domains ligated to the murine α3, transmembrane and cytoplasmic H2-Kb domains.
  • Expresses the chimeric HLA-B44 class I molecule on the surface of T and B cells.
  • Represents the human HLA-B44 supertype. The B*40:02 allele is a member of the B44-binding supertype, which encompasses a group of HLA B alleles sharing a binding specificity for peptides with E in position 2 and aliphatic/hydrophobic residues at the C-terminus. B61 is a serological designation. The B61 serological antigen is present on several B*40 subtypes (including B*40:02). With one possible exception, all alleles with the B61 serological antigen are expected to share the same main binding specificity as B*40:02, and hence have been defined as members of the B44 binding supertype.
  • Useful for infectious disease research, vaccine development and testing, safety and immunogenicity testing as well as research directed towards oncology and autoimmune disorders and syngeneic tumor studies utilizing C57BL/6-derived tumors.
  • Permits identification of epitopes restricted to the HLA-B44 supertype.
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Origin

The HLA-B44 model was generated by Pharmexa-Epimmune. The model was created by microinjecting a chimeric transgene combining a fragment of the genomic clone of the human HLA-B*4002 gene that included leader sequence, α1 and α2 domains ligated to a fragment of the murine H2-Kb gene containing the α3, transmembrane and cytoplasmic domains, all under the control of the murine H2-Kb promoter. This transgene was microinjected into C57BL/6 zygotes. The resultant mice from Founder Line B44.01 were on an inbred C57BL/6 background. The line was maintained by breeding hemizygotes to wild type C57BL/6NTac mice for 4 generations. Taconic received stock from Pharmexa-Epimmune in 2008. The line was embryo transfer derived by breeding C57BL/6NTac wild type females to hemizygous males and then was intracrossed to homozygosity. The colony is maintained through breeding of homozygous males to homozygous females. SNP profiles indicate that the strain is currently at 100% (NE10) on the C57BL/6NTac genetic background. In the production colony, wild type C57BL/6NTac females are bred to homozygous males on the C57BL/6NTac background to produce hemizygous pups on the C57BL/6NTac background. In addition, another production colony is breeding BALB/cAnNTac females with homozygous males on the C57BL/6NTAC background to product hemizygous CB6F1 pups, which are sold under model #9664.

Genetics

Species:Mouse
Strain Type:Congenic
Allele Type:Random Transgenic;Genetically Humanized
Coat Color:Black
Genetic Background:C57BL/6

Guides & Publications

Initial Publication: Depla E, Van der Aa A, Livingston BD, Crimi C, Allosery K, De Brabandere V, Krakover J, Murthy S, Huang M, Power S, Babé L, Dahlberg C, McKinney D, Sette A, Southwood S, Philip R, Newman MJ, Meheus L. (2008) Rational design of a multiepitope vaccine encoding T-lymphocyte epitopes for treatment of chronic hepatitis B virus infections. J Virol 82(1):435-50.

This publication does not describe the HLA-B44 strain, but the HLA-B44 strain was generated in a similar manner to that of the other transgenic HLA mouse strains described in the publication and may be used for similar types of experiments.

Applications & Therapeutic Areas

  • Autoimmune Disease
  • Immunology
  • Infectious Disease
  • Oncology & Immuno-Oncology
  • Safety Assessment
  • Vaccine Research
  • Inflammation

Transit, Housing & Welfare

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Packing Practices

Taconic standard practice is to recombine animals of different home cages and/or ages from a single model and sex during packing, except in specific cases where Taconic's animal welfare policy prohibits recombination due to aggression or other concerns.  When an order is fulfilled with animals from more than one week of birth, this standard practice results in animals from a range of birth weeks packed together in a single TTC. When an order is fulfilled with animals from genotyped models, this standard practice results in animals from different home cages packed together in a single TTC.

Customers who wish to keep animals from different weeks of birth separated should place orders with the special instruction "Divide and label by age."  Note that this special request can result in increased costs for additional Taconic Transit Cages, dividers and/or freight charges.

Taconic discourages other types of custom packing requests as they can have a negative impact on animal welfare. Learn more.

HLA-B44 (C57BL/6)

Conditions of Use for Taconic Transgenic Models™

Taconic Transgenic Models™ (Models) are produced and distributed under rights to patents and intellectual property licensed from various institutions. Taconic sells the Models to purchasers, grants to each purchaser a right under Taconic's rights in such licensed patents and intellectual property to use the purchased Model in consideration of purchasers' acknowledgement of and agreement to the Terms and Conditions for Taconic Models, Products and Services and the following terms of use:

  • Title to these Models and biological materials derived from them remains with Taconic.
  • The Models will be used for research purposes only.
  • The Models will not be bred or cross-bred except to obtain embryos or fetuses required for research purposes unless additional rights have been granted in writing by Taconic.
  • The Models and biological materials derived from them will not be distributed to third parties or used for commercial purposes.
  • Non-profit purchasers may not use this Model and/or biological materials derived from it in sponsored research or contract research studies unless it is purchased at the for-profit price.

Pricing - USD

Murine Pathogen Free (MPF) Health Standard

8911 Female

8911-F Genotype tg/wt

Age in WeeksQuantity 1 - 999
3 to 10$476.00

8911 Male

8911-M Genotype tg/wt

Age in WeeksQuantity 1 - 999
3 to 10$476.00

Pricing - EUR

Murine Pathogen Free (MPF) Health Standard

8911 Female

8911-F Genotype tg/wt

Age in WeeksQuantity 1 - 999
3 to 10€427,00

8911 Male

8911-M Genotype tg/wt

Age in WeeksQuantity 1 - 999
3 to 10€427,00

Pricing - USD Nonprofit

Murine Pathogen Free (MPF) Health Standard

8911 Female

8911-F Genotype tg/wt

Age in WeeksQuantity 1 - 999
3 to 10$275.00

8911 Male

8911-M Genotype tg/wt

Age in WeeksQuantity 1 - 999
3 to 10$275.00

Pricing - EUR Nonprofit

Murine Pathogen Free (MPF) Health Standard

8911 Female

8911-F Genotype tg/wt

Age in WeeksQuantity 1 - 999
3 to 10€247,00

8911 Male

8911-M Genotype tg/wt

Age in WeeksQuantity 1 - 999
3 to 10€247,00

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