| Model No. | Nomenclature | Genotype |
|---|---|---|
| 21546-F | B6;STOCK-Prkntm2Tmd | fl/fl |
| 21546-M | B6;STOCK-Prkntm2Tmd | fl/fl |
Floxed Parkin Mouse
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Overview
Nomenclature: B6;STOCK-Prkntm2Tmd
Model Sponsor: The Michael J. Fox Foundation
- This strain contains a conditional Prkn allele in which exon 7 is floxed
- The Prkn gene may be deleted in a tissue- or temporal-specific manner using viral delivery of Cre recombinase
- Mutations in the homologous PRKN gene are associated with early-onset Parkinson’s disease in humans
- NOTE: a number of tissue-specific and tamoxifen-sensitive Cre deleter mice were evaluated for compatibility with this line. Unfortunately, all of the Cre deleter lines evaluated uniformly deleted the Prkn gene in utero. As such, to avoid developmental compensation we recommend use of AAV-Cre to delete Parkin in regions of interest from adult animals. Please refer to references for additional information on using AAV-Cre to drive Parkin deletion.
Model #21546 contains the allele shown as “targeted allele” above. Cre-mediated recombination generates the allele shown as “mutant allele” above. Deletion of exon 7 results in a frame shift and premature termination of translation at a stop codon in exon 8. Use of viral-mediated cre rather than cre driver mice is recommended to prevent in utero deletion of Prkn and developmental compensation.
Figure excerpted from Von Coelln R, Thomas B, Savitt JM, Lim KL, Sasaki M, Hess EJ, Dawson VL, Dawson TM. Loss of locus coeruleus neurons and reduced startle in parkin null mice. Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10744-9. doi: 10.1073/pnas.0401297101. Epub 2004 Jul 12. PMID: 15249681; PMCID: PMC490005. Copyright (2004) National Academy of Sciences, U.S.A. Used with permission.
Availability
Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.
Ordering Note for Controls: Model #21546 WT littermate controls are not available for this model due to the homozygous mating format. Black 6 may be used as an approximate wild type control, but note that Model #21546 is not congenic on B6.
Origin
The floxed Prkn allele was generated by Lexicon Pharmaceuticals. The targeting vector included Prkn exon 7 flanked by loxP-sites and an FRT-flanked neomycin selection cassette. Targeting ES cells were injected into blastocysts. Chimeric mice were bred to protamine-cre mice to generate heterozygous pups containing one floxed and one deleted allele. Taconic received stock from the laboratory of Ted Dawson at Johns Hopkins University.
Genetics
Species: Mouse
Strain Type: Outbred or Mixed
Allele Type: Conditional Knockout
Coat Color: Various
Genetic Background: Mixed: Line was developed on a C57BL/6J background. Line is currently on a mixed genetic background (83.48-84.24% of the genetic background is C57BL/6)
Guides & Publications
Von Coelln R, Thomas B, Savitt JM, Lim KL, Sasaki M, Hess EJ, Dawson VL, Dawson TM. Loss of locus coeruleus neurons and reduced startle in parkin null mice. Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10744-9.
Shin JH, Ko HS, Kang H, Lee Y, Lee YI, Pletinkova O, Troconso JC, Dawson VL, Dawson TM. PARIS (ZNF746) repression of PGC-1α contributes to neurodegeneration in Parkinson's disease. Cell. 2011 Mar 4;144(5):689-702.
Applications & Therapeutic Areas
- Neuroscience
- Parkinson’s disease
Transit, Housing & Welfare
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Diet
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Licensing
Label License Agreement and Conditions of Use for Floxed Parkin Mouse (Model #21546)
The Floxed Parkin Mouse ("Model") is produced and distributed under rights to patents and/or intellectual property owned by or licensed to Lexicon Pharmaceuticals ("Lexicon") and Taconic. Taconic has rights to breed and distribute the Model. The Michael J. Fox Foundation ("MJFF") is sponsoring the sales of the Model for the benefit of the scientific community. Taconic, MJFF, and Lexicon permit the sale of such Model to each purchaser that agrees to the Terms and Conditions of the Sale and following terms of use:
- Title to the Model, any progeny and biological materials derived from the foregoing remains with Lexicon.
- The Model may be bred or crossbred by purchaser or by Taconic for purchaser.
- Purchaser shall inform MJFF of any crossbred animal generated ("Modified Progeny"), shall at MJFF's election deposit breeding pairs of each Modified Progeny with Taconic and shall permit Taconic to distribute the Modified Progeny on behalf of MJFF to non-profit institutions or for-profit institutions without any compensation to purchaser on terms similar to the terms and conditions by which purchaser received the Model. Purchaser assumes all liability for any claim or demand that Modified Progeny created by purchaser infringes on the intellectual property rights of any third party.
- The Model and progeny may be used by non-profit institutions or for-profit institutions for research purposes only.
- The Model, progeny or biological materials will not be used for commercial purposes.
- The Model, progeny, and biological materials derived from them will not be distributed to any third party (except to send tissues to a collaborator or for fee-for-service staining or analysis work).
- Purchaser assumes all risk from using the Model, progeny and biological materials.
- To the extent permissible by law, purchaser indemnifies, defends, and holds harmless MJFF and Lexicon from any claim or demand that purchaser's use or transfer of the Model, progeny, or biological materials has caused harm, damage or liability.
Pricing - USD
Murine Pathogen Free (MPF) Health Standard
21546 Female
21546-F Genotype fl/fl
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $140.00 |
21546 Male
21546-M Genotype fl/fl
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | $140.00 |
Pricing - EUR
Murine Pathogen Free (MPF) Health Standard
21546 Female
21546-F Genotype fl/fl
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €129,00 |
21546 Male
21546-M Genotype fl/fl
| Age in Weeks | Quantity 1 - 999 |
| 3 to 10 | €129,00 |
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