A humanized immune system (HIS) mouse supporting both myeloid and lymphoid cells and designed for more accurate assessment of antibody-based drugs.

Nomenclature: NOD.Cg-Fcgr2b^tm1Ttk Fcer1g^tm1Rav Prkdc^scid Il2rg^tm1Sug Tg(SV40/HTLV-IL3,CSF2)10-7Jic/JicTac

For researchers who want access to live inventory for immediate delivery, the FcResolv huNOG-EXL SA (Standard Access) is the ideal model, particularly for experiments with shorter timelines or studies that must start as soon as possible.

Need a longer experimental window? Compare the Standard Access and Early Access models to learn which is right for your project.

• The FcResolv® huNOG-EXL is a HIS mouse made by engrafting human CD34+ hematopoietic stem cells (HSCs) in the FcResolv NOG-EXL strain, available as an inventoried, study-ready product
• All mice shipped meet a specified QC standard of ≥25% hCD45 in peripheral blood at 10 weeks post-engraftment
• In most cases, FcResolv huNOG-EXL mice average >40% human cells in blood
• Humanized immune system mice require special housing and husbandry. We recommend reviewing the the comprehensive document Licensing, Care & Resources for Taconic's Humanized Immune System Models and the Video: Care of HIS mice for experimental success prior to ordering. We strongly recommend that customers schedule a complimentary consultation with a Field Application Scientist prior to ordering so as to maximize experimental success.

Not sure which FcResolv huNOG-EXL product to choose? See below to view our in-depth comparison chart. Taconic's PhD Field Applications Scientists can also help you determine which model is best suited for your study.

FcResolv NOG-EXL Characteristics 

• The FcResolv® NOG-EXL is a super immunodeficient NOG mouse that expresses human GM-CSF and IL-3 cytokines and has a further reduction in residual murine immune activity via ablation of mouse Fc gamma receptors (FcγRs) to reduce murine immune cell interactions with antibody-based drugs
• This model can be used in a similar fashion to the NOG-EXL mouse, including for xenograft and immune system humanization experiments
• Murine FcγRs can confound experiments in several ways, leading to false positive or negative results. Removing murine FcγRs provides improved accuracy for efficacy assessment of antibody-based therapies which include an Fc domain.
  • Lacks all murine FcγRs, including the FcγRI, IIB, III and IV types, along with the high affinity FcεRI receptor. The low affinity FcεRII receptor remains present

• The NOG-EXL model was designed to express relatively low cytokine levels, which maintains the long-term stability of engraftment by preventing the exhaustion of the stem cell pool. This stability makes it a flexible platform for the generation of humanized mice.
  • Supports myeloid lineage engraftment through human IL-3 and GM-CSF cytokine expression
  • More efficient CD34+ human hematopoietic stem cell (HSC) engraftment
  • Higher levels of myeloid cell differentiation following human HSC engraftment
  • Low cytokine expression and successful long-term HSC engraftment
  • NOG-EXL mice are a superior host for acute myeloid leukemia (AML) patient-derived xenografts. 
• Research applications include the generation of humanized mouse models, oncology, infectious disease, and regenerative medicine

Which FcResolv huNOG-EXL is right for you?

Orders by weight: Taconic cannot accept orders by weight for this model. Please note that shipments may contain animals with a larger weight variation.

Availability

• Taconic maintains an inventory of female FcResolv huNOG-EXL SA mice engrafted as juveniles and FACS tested at 10 weeks post-engraftment.
• Orders with a specification regarding number of mice per donor are subject to availability and are not guaranteed, as orders may need to be adjusted at time of packing.

NOTE: Please contact Taconic prior to your first order for a review of unpacking instructions. Tattoo numbers may repeat between engraftment lots. Individual ID number is determined through examination of the tattoo PLUS the box and section location.

Lifespan: All myeloid-supportive HIS mice have limited lifespans due to a range of outcomes including anemia, thrombocytopenia, macrophage activation syndrome and hemophagocytic lymphohistiocytosis. The huNOG-EXL has the longest demonstrated lifespan compared to competing models, but this varies by donor and can be impacted by environmental and experimental factors. While Taconic has observed that huNOG-EXL mice can often survive for 25+ weeks post-engraftment (WPE), researchers should plan for studies to be complete by 23-26 WPE.

Origin

FcResolv NOG-EXL mice engrafted with human CD34+ hematopoietic stem cells (HSCs) stably develop extensive cell lineages as early as 6 to 8 weeks post-injection. Both myeloid and lymphoid lineage cells are present in peripheral blood, bone marrow, thymus and spleen and non-lymphoid tissue including lung and liver. While not all human immune cell types have been exhaustively characterized in these mice, extensive myeloid differentiation including granulocyte lineages (basophil, neutrophil and mast cells) are evident in blood and tissues. Antigen presenting cells (dendritic cells and macrophages) are also increased in frequency, both in blood and spleen.

FcResolv® refers to a United States trademark registration.